Oxidative DNA damage induced by activation of polychlorinated biphenyls (PCBs): implications for PCB-induced oxidative stress in breast cancer

Chemical Research in Toxicology
G G OakleyR C Gupta

Abstract

We have previously reported that mono- and dichlorinated biphenyls (PCBs) can be metabolized to dihydroxy compounds and further oxidized to reactive metabolites which form adducts with nitrogen and sulfur nucleophiles including DNA [Amaro et al. (1966) Chem. Res. Toxicol. 9, 623-629; Oakley et al. (1996) Carcinogenesis 17, 109-114]. The former studies also demonstrated that during the metabolism of PCBs superoxide may be produced. We have therefore examined the abilities of PCB metabolites to induce free radical-mediated oxidative DNA damage using a newly developed, highly sensitive, 32P-postlabeling assay for 8-oxode-oxyguanosine (8-oxodG) [Devanaboyina, U., and Gupta, R. (1996) Carcinogenesis 17, 917-924]. The incubation of 3,4-dichloro-2'5'-dihydroxybiphenyl (100 microM) with calf thymus DNA (300 micrograms/microL) in the presence of the breast tissue and milk-associated enzyme, lactoperoxidase (10 mU/mL), and H2O2 (0.5 mM) resulted in a significant increase in free radical-induced DNA damage (253 8-oxodG/10(6) nucleotides) as compared to vehicle-treated DNA (118 8-oxodG/10(6) nucleotides). Substituting CuCl(2) (100 microM) for lactoperoxidase/H2O2, however, resulted in a substantial increase in 8-oxodG content (2669 8-oxodG...Continue Reading

References

Jan 1, 1992·Critical Reviews in Toxicology·T J Monks, S S Lau
Jan 6, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P F Hollenberg
Jan 1, 1990·Critical Reviews in Toxicology·E M SilberhornL W Robertson
Jan 1, 1990·Free Radical Biology & Medicine·J G Liehr, D Roy
Jan 1, 1989·Chemico-biological Interactions·K AgarwalG Talukder
Jan 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P M QuinseyJ T Ahokas
Jan 1, 1993·Free Radical Biology & Medicine·C C Winterbourn

❮ Previous
Next ❯

Citations

Dec 6, 2012·Cancer Letters·Gabriele Ludewig, Larry W Robertson
Dec 26, 2003·Biophysical Journal·Chiara CiaccioMassimo Coletta
Feb 14, 2003·Toxicology and Applied Pharmacology·Parvaneh EspandiariLarry W Robertson
Aug 16, 2003·Toxicology Letters·P ApostoliM Manno
Oct 3, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·K HilscherovaJ P Giesy
Dec 25, 2008·Chemical Research in Toxicology·Orarat WangpraditGregor Luthe
Jul 28, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Margarita C Currás-Collazo
Feb 24, 2001·Antioxidants & Redox Signaling·C B Ambrosone
Feb 27, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·A SrinivasanG Ludewig
Sep 3, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yueming ZhuNukhet Aykin-Burns
Jul 4, 2012·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalSajal Gupta
Apr 10, 2002·Environmental Health Perspectives·Kathryn Jessen-EllerCarol L Reinisch
Feb 9, 2002·Environmental Health Perspectives·David O CarpenterDavid C Spink
Jun 10, 1998·Environmental Health Perspectives·V J Cogliano
May 17, 2006·Reviews on Environmental Health·David O Carpenter
Mar 28, 2009·International Journal of Molecular Sciences·Harini S AiyerRamesh C Gupta
Nov 28, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·A StronatiD Bizzaro
Dec 25, 2015·Chemical Research in Toxicology·Diana Montes-GrajalesJesus Tadeo Olivero-Verbel
Sep 24, 2015·Environmental Science and Pollution Research International·Orarat WangpraditGregor Luthe
Sep 25, 2015·Environmental Science and Pollution Research International·Orarat WangpraditGregor Luthe
Dec 24, 2015·BMC Systems Biology·Mona YousofshahiSoha Hassoun
Sep 9, 2009·Environment International·Srivani RavooriRamesh C Gupta
May 12, 2009·Toxicology Letters·Oddvar MyhreFrode Fonnum
Feb 24, 2009·Free Radical Biology & Medicine·Wendy A SpencerRamesh C Gupta
Sep 18, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Job C TharappelHoward P Glauert
Jan 6, 2009·Environmental Toxicology and Pharmacology·Howard P GlauertBrett T Spear
May 3, 2008·Environmental Toxicology and Pharmacology·Gabriele LudewigLarry W Robertson
Apr 29, 2008·Environmental Toxicology and Pharmacology·Catherine MaddoxGabriele Ludewig

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.