PMID: 9438559Jan 23, 1998Paper

Oxidative inactivation of gastric peroxidase by site-specific generation of hydroxyl radical and its role in stress-induced gastric ulceration

Free Radical Biology & Medicine
D DasR K Banerjee

Abstract

We have shown earlier that restraint-cold stress-induced gastric ulceration in rats is caused by metal ion-dependent generation of hydroxyl radical (OH.) and oxidative inactivation of the gastric peroxidase (GPO), an important H2O2 scavenging enzyme. To study the mechanism of the oxidative damage of GPO, the purified enzyme was exposed to an OH. generating system containing Cu2+, ascorbate, and H2O2. Kinetic studies indicate that the enzyme is inactivated in a time-dependent process showing saturation with respect to Cu2+ concentration. The enzyme specifically requires Cu2+ and is not inactivated by the same concentration of Fe2+, Mn2+, or Zn2+. Sensitivity to catalase indicates the critical role of H2O2 in the inactivation. Inactivation is insensitive to superoxide dismutase, suggesting no role of superoxide. The rate of inactivation is not increased in D2O excluding the involvement of singlet oxygen in the process. However, OH. scavengers such as benzoate or mannitol cannot prevent inactivation. The results indicate a plausible generation of OH. within the enzyme molecule as the cause of inactivation. Fragmentation of peptide linkage or intramolecular crosslinking, gross change of tertiary structure, or change in intrinsic tr...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C N OliverR A Floyd
Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M B YimE R Stadtman
Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Jan 1, 1990·Methods in Enzymology·R E Pacifici, K J Davies
Mar 15, 1989·Biochemical Pharmacology·M BhattacharjeeR K Banerjee
Mar 1, 1989·The Biochemical Journal·P G WinyardD R Blake
Aug 15, 1988·Analytical Biochemistry·J K GradyD C Harris
May 1, 1986·Archives of Biochemistry and Biophysics·B Halliwell, J M Gutteridge
Oct 15, 1986·European Journal of Biochemistry·S K De, R K Banerjee
Dec 1, 1987·Digestive Diseases and Sciences·G PihanS Szabo
Feb 1, 1986·Gastroenterology·M A PerryD N Granger
Mar 30, 1971·Biochemistry·D E Schmidt, F H Westheimer
Apr 1, 1972·Journal of Pharmaceutical Sciences·S M Blaug, B Hajratwala
Aug 21, 1984·Biochimica Et Biophysica Acta·S K De, R K Banerjee
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R L LevineE R Stadtman
Jan 1, 1995·The Biochemical Journal·D DasR K Banerjee
Nov 15, 1993·The Biochemical Journal·U BandyopadhyayR K Banerjee
Aug 25, 1993·Molecular and Cellular Biochemistry·D Das, R K Banerjee
May 1, 1993·Biochemical Society Transactions·B E Hedlund, P E Hallaway
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M ChevionN Kitrossky
Jan 1, 1997·Free Radical Biology & Medicine·D DasR K Banerjee
Jul 15, 1997·Biochemical Pharmacology·U BandyopadhyayR K Banerjee
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·B D SanterM F Wehner

❮ Previous
Next ❯

Citations

Feb 24, 2001·Journal of Physiology and Biochemistry·O Martínez-AugustínF Sánchez de Medina
Sep 25, 2002·Life Sciences·Toshiaki MiuraYukio Fujimoto
Nov 2, 2005·Inflammopharmacology·Tohru KotaniKoji Takeuchi
Sep 4, 2009·Journal of Hematology & Oncology·Subhash PadhyeFazlul H Sarkar
May 4, 2011·World Journal of Biological Chemistry·Olena V BogdanovaLudmyla I Ostapchenko
Jan 14, 2009·Phytotherapy Research : PTR·Pallab MaityUday Bandyopadhyay
Feb 9, 2005·Chemico-biological Interactions·Sanae Muraoka, Toshiaki Miura
Apr 15, 2005·Redox Report : Communications in Free Radical Research·Shinjiro MiyakeMasaichi-Chang-il Lee
May 29, 2015·Biotechnology, Biotechnological Equipment·Ivan KindekovKrassimira Idakieva
Oct 26, 2002·Chemico-biological Interactions·Giuseppe GalatiPeter J O'Brien
Mar 27, 2001·Free Radical Research·T MiuraY Fujimoto
Mar 4, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mira PopovicSvetlana Trivic
May 8, 2004·Free Radical Research·Sanae Muraoka, Toshiaki Miura
Jan 17, 2003·The Journal of Biological Chemistry·Kaushik BiswasRanajit K Banerjee
Jul 30, 2019·Journal of Food Biochemistry·Najmeh Hadizadeh Shirazi
Jun 2, 2021·Cell & Bioscience·Jia-Yi HouJi-Min Cao
Sep 14, 2006·Chemical Reviews·Roxana Ciochina, Robert B Grossman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.