Oxidative metabolism of linoleic acid by human leukocytes

Biochemical and Biophysical Research Communications
O ReinaudD Mansuy


Upon incubation with human leukocytes, [1-14C] linoleic acid is almost exclusively transformed into 13-hydroxy-9Z, 11E-octadecadienoic acid (13-HODE) if the linoleic acid concentration is lower than 50 microM. Identification of 13-HODE was done by GLC-MS at the level of its methyl ester, trimethylsilyl ether and by comparison with authentic 13-HODE in two different HPLC systems. Analysis of the products by chiral phase HPLC shows that 13(S)-hydroxy-9Z, 11E-octadecadienoic acid is by far the major metabolite formed by human leukocytes. Comparison of reactions performed with intact or lyzed cells suggests that the formation of 13(S)-HODE by human leukocytes occurs in two steps, a dioxygenation catalyzed by a 15-lipoxygenase and a reduction of intermediate 13-HPODE by a glutathione-dependent peroxidase.


Jan 1, 1977·Progress in the Chemistry of Fats and Other Lipids·G A VeldinkJ Boldingh
Oct 23, 1985·Biochimica Et Biophysica Acta·M ClaeysD H Nugteren
Jan 1, 1988·Progress in Lipid Research·A A SpectorS A Moore
Jul 31, 1987·Biochemical and Biophysical Research Communications·B N SettyM J Stuart
Sep 4, 1987·Biochimica Et Biophysica Acta·D H Nugteren, G A Kivits
Sep 25, 1987·Biochimica Et Biophysica Acta·P WalstraJ F Vliegenthart
Oct 29, 1987·Biochemical and Biophysical Research Communications·B N Yamaja SettyM J Stuart
May 29, 1986·Biochemical and Biophysical Research Communications·M HayakawaT Ozawa
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P S AisenG Weissmann
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Mar 15, 1984·European Journal of Biochemistry·H KühnS M Rapoport

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