PMID: 9555005May 23, 1998Paper

Oxidative modification of HDL3 in vitro and its effect on PLTP-mediated phospholipid transfer

Biochimica Et Biophysica Acta
J HuuskonenC Ehnholm

Abstract

The oxidation of HDL3 by Cu(II) and its effect on the ability of these particles to act as phospholipid acceptors in human plasma phospholipid transfer protein (PLTP)-mediated lipid transfer were investigated. Oxidation of HDL3 was monitored by measuring the following parameters: (i) formation of conjugated dienes, (ii) production of thiobarbituric acid reactive substances (TBARS), (iii) decrease in reactive lysine and (iv) tryptophan residues, (v) change in particle charge and (vi) diameter, and (vii) oligomerisation of apoA-I and apoA-II. Formation of conjugated dienes was the parameter responding to the oxidative treatment with the fastest kinetics. The appearance of TBARS and modification of apolipoprotein tryptophan residues were detected simultaneously but required higher Cu(II) concentrations for maximal kinetics. Cross-linking of the major protein constituents of HDL3, apoA-I and apoA-II, represented later steps of the oxidation process. Further, the oxidative modification was accompanied by a progressive change in HDL3 particle charge and a minor increase in particle diameter. PLTP-mediated phospholipid transfer to the oxidized particles was investigated using an assay measuring the transfer of fluorescent, pyrene-labe...Continue Reading

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Citations

Mar 20, 2001·Atherosclerosis·J HuuskonenC Ehnholm
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Pirkko J PussinenErnst Malle
Aug 21, 2010·Journal of Lipid Research·Niko L SetäläMatti Jauhiainen
Nov 18, 2003·Journal of Lipid Research·Sarah SigginsChristian Ehnholm
Nov 13, 1998·Current Opinion in Lipidology·M NavabA M Fogelman
Jul 11, 2006·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Giuseppe Danilo NorataAlberico Luigi Catapano
Feb 27, 2007·Biochimica Et Biophysica Acta·Rhoderick E Brown, Peter Mattjus

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