PMID: 9165300Jan 1, 1997Paper

Oxidative modifications of apoB-100 by exposure of low density lipoproteins to HOCL in vitro

Free Radical Biology & Medicine
C YangC V Smith

Abstract

Although the products of oxidation of the lipid components of LDL have been studied extensively, much less is known about the specific products of oxidative modification of the apoprotein. We reacted native LDL and LDL that had been treated with HOCl with 2,4-dinitrophenylhydrazine (DNPH), delipidated and trypsinized the protein, and analyzed the products by HPLC. Although tryptic digests of native LDL and LDL oxidized by limited quantities of HOCl showed similar patterns by HPLC with detection at 220 nm, oxidized LDL showed several discrete peaks at 365 nm, which is characteristic of hydrazones formed with aldehydes and ketones, commonly termed protein carbonyls. Native LDl showed no peaks in the chromatograms at 365 nm. Peptides absorbing at 365 nm were isolated by HPLC and characterized. In most cases, the probable sites of modification on the peptides could be implied by failure of an anticipated amino acid to appear in the expected sequence. Of the 14 peptides isolated and characterized to date, eight peptides contained Cys residues. In other peptides, Lys, Trp, and Met were identified as amino acid residues apparently modified by HOCl treatment of LDL. Thirteen of the peptides identified are from trypsin-releasable peptid...Continue Reading

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Citations

Jan 1, 1997·Free Radical Biology & Medicine·C Y YangC V Smith
Apr 30, 2011·The Journal of Biological Chemistry·Tina M DreadenBridgette A Barry
Aug 19, 2011·Biological Chemistry·Hsin-Hung ChenChao-Yuh Yang
Nov 13, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jochen WeigeleReinhard Hilbig
Oct 31, 2012·Molecular Aspects of Medicine·Niki ChondrogianniEfstathios S Gonos
Jan 19, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jonatas Zeni KlafkePaulo Ricardo Nazário Viecili
Mar 26, 2015·Applied Biochemistry and Biotechnology·Zehra Tavsan, Hulya Ayar Kayali
May 15, 2007·Journal of Lipid Research·John W NewmanGregory C Shearer
Aug 8, 2002·Luminescence : the Journal of Biological and Chemical Luminescence·A Aspée, E A Lissi
Apr 12, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Chao-yuh YangCharles V Smith
Jul 26, 2018·Journal of Biomolecular Structure & Dynamics·Fakhra Amin, Bilqees Bano
Aug 1, 2006·Pathophysiology of Haemostasis and Thrombosis·Elisabeth KollerFranz Koller
Jan 5, 2000·Physiological Reviews·G J Schroepfer
Oct 4, 2006·Journal of Lipid Research·J GloerichS Ferdinandusse
May 16, 2006·Biochimica Et Biophysica Acta·Ernst MalleMichael J Davies

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