Oxidative monensin metabolism and cytochrome P450 3A content and functions in liver microsomes from horses, pigs, broiler chicks, cattle and rats

Journal of Veterinary Pharmacology and Therapeutics
Carlo NebbiaMonica Carletti

Abstract

The oxidative metabolism of monensin, an ionophore antibiotic extensively used in veterinary practice as a coccidiostat and a growth promoter, was studied in hepatic microsomal preparations from horses, pigs, broiler chicks, cattle and rats. As assayed by the measurement of the amount of the released formaldehyde, the rate of monensin O-demethylation was nearly of the same order of magnitude in all species, but total monensin metabolism, which was estimated by measuring the rate of substrate disappearance by a high-performance liquid chromatography (HPLC) method, was highest in cattle, intermediate in rats, chicks and pigs, and lowest in horses. When expressed as turnover number (nmol of metabolized monensin/min nmol cytochrome P450-1), the catalytic efficiency (chick > cattle > pig approximately rat > horse) was found to correlate inversely with the well known interspecies differences in the susceptibility to the toxic effects of the ionophore, which is characterized by an oral LD50 of 2-3 mg/kg bodyweight (bw) in horses, 50-80 mg/kg bw in cattle and 200 mg/kg bw in chicks. Chick and cattle microsomes also displayed both the highest catalytic efficiency toward two P450 3A dependent substrates (erythromycin and triacetyloleando...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1993·Journal of Veterinary Pharmacology and Therapeutics·P GaltierC Eeckhoutte
Jan 1, 1996·Canadian Journal of Physiology and Pharmacology·M CouletP Galtier
Feb 7, 1998·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·L CeppaC Nebbia
May 20, 1999·Research in Veterinary Science·A Anadón, L Reeve-johnson
Nov 24, 1999·Pharmacology & Toxicology·M T Skaanild, C Friis

❮ Previous
Next ❯

Citations

Jan 11, 2011·Toxicology and Applied Pharmacology·J L C M DorneJ Fink-Gremmels
Mar 21, 2003·AAPS PharmSci·Arthur L Craigmill, Kristy A Cortright
Oct 25, 2011·Avian Diseases·Guillermo ZavalaLouise Dufour-Zavala
Jul 7, 2011·American Journal of Veterinary Research·Allison J StewartJenny Springfield
Feb 3, 2015·Journal of Biomolecular Structure & Dynamics·Yousef Altaher, Mahmoud Kandeel
Oct 16, 2004·Letters in Applied Microbiology·Y S JungD J Nisbet
Nov 7, 2006·Journal of Veterinary Pharmacology and Therapeutics·K A Cortright, A L Craigmill
Oct 19, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Bruno A RochaAnderson R M de Oliveira
Jul 8, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Michela CantielloCarlo Nebbia
Nov 13, 2008·Journal of Veterinary Pharmacology and Therapeutics·J HenriM Laurentie
Nov 11, 2010·Journal of Veterinary Pharmacology and Therapeutics·L L ZhangS X Jiang
Jan 11, 2019·Chembiochem : a European Journal of Chemical Biology·Marius Grote, Frank Schulz
Oct 23, 2010·Drug Testing and Analysis·James P ScarthTiia Kuuranne
Mar 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lidia RadkoAndrzej Posyniak
Sep 25, 2019·Acta Veterinaria Hungarica·Orsolya PalóczGyörgy Csikó
Dec 25, 2019·Scientific Reports·Mery GiantinMauro Dacasto
Jul 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lisa KotthoffMatthias Koch
Sep 8, 2020·Journal of Veterinary Pharmacology and Therapeutics·Alberto NassiMery Giantin
Mar 31, 2021·Australian Veterinary Journal·P GrayUNKNOWN Australian Veterinary Association Ltd and Animal Medicines Australia
May 20, 2021·Journal of Veterinary Pharmacology and Therapeutics·Kaixiang ZhouLingli Huang
Apr 18, 2008·European Journal of Pharmacology·Johanna Fink-Gremmels

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.