PMID: 6165388May 13, 1981Paper

Oxidative phosphorylation by membrane vesicles from Bacillus alcalophilus

Biochimica Et Biophysica Acta
A A GuffantiT A Krulwich

Abstract

ADP and Pi-loaded membrane vesicles from t-malate-grown Bacillus alcalophilus synthesized ATP upon energization with ascorbate/N,N,N',N'-tetra-methyl-P-phenylenediamine. ATP synthesis occurred over a range of external pH from 6.0 to 11.0, under conditions in which the total protonmotive force delta-mu-H+ was as low as -30 mV. The phosphate potentials (delta Gp) were calculated to be 11 and 12 kcal/mol at pH 10.5 and 9.0, respectively, whereas the delta-mu-H+ values in vesicles at these two pH values were quite different (-40 +/- 20 mV at pH 10.5 and -125 +/- 20 mV at pH 9.0). ATP synthesis was inhibited by KCN, gramicidin, and by N,N1-dicyclohexylcarbodiimide. Inward translocation of protons, concomitant with ATP synthesis, was demonstrated using direct pH monitoring and fluorescence methods. No dependence upon the presence of Na+ or K+ was found. Thus, ATP synthesis in B. alcalophilus appears to involve a proton-translocating ATPase which functions at low delta-mu-H+.

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Citations

Dec 1, 1989·Journal of Bioenergetics and Biomembranes·V P Skulachev
Dec 1, 1989·Journal of Bioenergetics and Biomembranes·T A Krulwich, A A Guffanti
Jan 1, 1986·The Journal of Membrane Biology·T A Krulwich
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R D AstumianH V Westerhoff
Jul 23, 1986·Biochimica Et Biophysica Acta·P A DibrovM L Verkhovskaya
Jan 1, 1988·Critical Reviews in Microbiology·T A KrulwichA A Guffanti
Aug 15, 1989·European Journal of Biochemistry·A L SemeykinaK M Chumakov
Aug 3, 1987·European Journal of Biochemistry·E C Slater
Jan 1, 1982·Annals of the New York Academy of Sciences·T A Krulwich, A A Guffanti
Dec 1, 1986·Journal of Bacteriology·W H Nitschmann, G A Peschek
Jan 1, 1992·Archives of Biochemistry and Biophysics·B J Efiok, D A Webster

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