PMID: 11909681Mar 23, 2002Paper

Oxidative phosphorylation enzyme complexes in caloric restriction

Experimental Gerontology
Abdullah OlgunTurker Kutluay

Abstract

Free radicals, generated especially by electron leakage from mitochondrial electron transport chain (ETC), are accepted as one of the possible causes of aging. Long-term caloric restriction (CR) is known to increase the species specific average and maximum life spans. Thus it provides a means for investigating mechanisms of aging. There is evidence suggesting a decrease in the free radical production with CR. In this study, Blue-Native PAGE (BN-PAGE) technique was used to investigate the effect of CR on the oxidative phosphorylation enzyme complexes. Of the total 30 female Swiss Albino balb/c mice, 15 were used as control and the other 15 as CR group. Alternate day feeding regimen was used in the CR group for 66 weeks beginning at the end of 3rd month. In the control group, 5 (33.3%) mice died, 3 (20%) of them of breast cancer, 2 (13.3%) of unknown causes and no death cases were observed in the CR group during the study. BN-PAGE was performed on the extracts from brain mitochondrial fractions. Complexes II and V were excluded from the study due to some analytical limitations. No difference was found in the levels of complexes I and III between the groups. In the CR group, complex IV level was found increased and the ratio of co...Continue Reading

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Citations

Mar 8, 2005·Neurobiology of Aging·Nilay V PatelCaleb E Finch
Jun 29, 2004·The Journal of Nutritional Biochemistry·Oscar LamasAmelia Marti
Jul 9, 2013·Experimental Gerontology·Holly M Brown-Borg, Sharlene Rakoczy
Apr 27, 2007·Annals of the New York Academy of Sciences·Abdullah Olgun, Serif Akman
May 4, 2007·Neurochemical Research·A R Palaniappan, A Dai
Jul 12, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Barry Drew, Christiaan Leeuwenburgh
Aug 9, 2020·International Journal of Molecular Sciences·Yongde LuoWeiqin Lu
Feb 18, 2011·Cancer Prevention Research·Donna K CarverGustavo C Rodriguez

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