Oxidative Stability of Protease Treated Peanut with Reduced Allergenicity

Foods
Jianmei YuNona Mikiashvili

Abstract

Oxidative stability and allergenicity are two major concerns of peanuts. This study evaluated the impact of protease treatment of peanuts on its oxidative stability during storage. The raw and dry-roasted peanut kernels were hydrolyzed with Alcalase solution at pH 7.5 for 3 h. The contents of Ara h 1, Ara h 2, and Ara h 6 in peanuts were determined before and after enzyme treatment by a sandwich ELISA. After drying, the samples were packed in eight amber glass jars and stored at 37 °C for 1-8 weeks. Controls are untreated raw and dry-roasted peanuts packed and stored in the same way as their treated counterparts. Samples were taken biweekly to determine peroxide value (PV) and thiobarbituric acid reactive substances (TBARS) as indicators of oxidation (n = 3), and to determine antioxidant activity. Alcalase treatment reduced intact major allergens Ara h 1, Ara h 2, and Ara h 6 by 100%, 99.8%, and 85%, respectively. The PVs of Alcalase-treated raw and roasted peanuts was lower than those of untreated (p < 0.05) over the 8-week storage. The TBARS of Alcalase-treated raw peanuts were slightly higher than that of untreated (p < 0.05), but the TBARS of Alcalase-treated dry-roasted peanuts were slightly but significantly lower than th...Continue Reading

References

May 23, 2001·Nutrition Reviews·P M Kris-EthertonT D Etherton
Jul 6, 2010·Peptides·Bahareh H Sarmadi, Amin Ismail
Sep 14, 2011·International Archives of Allergy and Immunology·Beatriz CabanillasJesús F Crespo
Oct 2, 2012·Journal of Proteome Research·Christine M HeblingMelinda A McFarland
Jul 6, 2013·Nutrition Research·Jennette Palcic MorenoJohn P Foreyt
Nov 22, 2013·The New England Journal of Medicine·Ying BaoCharles S Fuchs
May 13, 2014·Allergy·B I NwaruUNKNOWN EAACI Food Allergy and Anaphylaxis Guidelines Group
Jun 6, 2014·The American Journal of Clinical Nutrition·Chandra L Jackson, Frank B Hu
Aug 20, 2014·Journal of Medicinal Food·Ying Li, Jianmei Yu
Aug 26, 2014·Jundishapur Journal of Microbiology·Alireza OstadrahimiSepideh Mahluji
Aug 1, 2011·Food Chemistry·Jianmei YuSoheila Maleki
Jun 4, 2015·The Journal of Allergy and Clinical Immunology·Arnd PetersenUta Jappe
Jan 16, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tang-Bin ZouEn-Qin Xia
Mar 8, 2016·International Journal of Epidemiology·Tannaz EslamparastReza Malekzadeh
Dec 1, 2017·Journal of Food Science and Technology·M Abbas AliAhmadilfitri Md Noor
Apr 24, 2018·Molecular Immunology·Chiara Palladino, Heimo Breiteneder
Jan 16, 2019·JAMA Network Open·Ruchi S GuptaKari C Nadeau
Sep 23, 2019·BMJ Nutrition, Prevention & Health·Xiaoran LiuDeirdre K Tobias

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Methods Mentioned

BETA
Protein Assay
ELISA
electrophoresis
ELISAs

Software Mentioned

SAS

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