Oxidative Stickland reactions in an obligate aerobic organism - amino acid catabolism in the Crenarchaeon Sulfolobus solfataricus

The FEBS Journal
Helge StarkDietmar Schomburg

Abstract

The thermoacidophilic Crenarchaeon Sulfolobus solfataricus is a model organism for archaeal adaptation to extreme environments and renowned for its ability to degrade a broad variety of substrates. It has been well characterised concerning the utilisation of numerous carbohydrates as carbon source. However, its amino acid metabolism, especially the degradation of single amino acids, is not as well understood. In this work, we performed metabolic modelling as well as metabolome, transcriptome and proteome analysis on cells grown on caseinhydrolysate as carbon source in order to draw a comprehensive picture of amino acid metabolism in S. solfataricus P2. We found that 10 out of 16 detectable amino acids are imported from the growth medium. Overall, uptake of glutamate, methionine, leucine, phenylalanine and isoleucine was the highest of all observed amino acids. Our simulations predict an incomplete degradation of leucine and tyrosine to organic acids, and in accordance with this, we detected the export of branched-chain and aromatic organic acids as well as amino acids, ammonium and trehalose into the culture supernatants. The branched-chain amino acids as well as phenylalanine and tyrosine are degraded to organic acids via oxid...Continue Reading

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Citations

Jan 10, 2018·Frontiers in Microbiology·Julian QuehenbergerOliver Spadiut
Jan 29, 2019·Frontiers in Microbiology·Lisa F BischofSonja-Verena Albers
Jun 11, 2019·Archaea : an International Microbiological Journal·Anna M VetterMeina Neumann-Schaal
Jan 22, 2021·FEMS Microbiology Reviews·April M LewisRobert M Kelly
Mar 21, 2021·Applied and Environmental Microbiology·Jens C BenninghoffBettina Siebers

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