Oxidative Stress and Aberrant Programmed Cell Death Are Associated With Pollen Abortion in Isonuclear Alloplasmic Male-Sterile Wheat

Frontiers in Plant Science
Zihan LiuXiyue Song

Abstract

Cytoplasmic male sterility is crucial for the utilization of hybrid heterosis and it possibly occurs in parallel with tapetal programmed cell death (PCD) and oxidative metabolism responses. However, little is known about the mechanisms that underlie pollen abortion in wheat. Therefore, we obtained two isonuclear alloplasmic male sterile lines (IAMSLs) with Aegilops kotschyi and Ae. juvenalis cytoplasm. Compared with the maintainer line, cytochemical analyses of the anthers demonstrated that the IAMSLs exhibited anomalous tapetal PCD and organelles, with premature PCD in K87B1-706A and delayed PCD in Ju87B1-706A. We also found that the dynamic trends in reactive oxygen species (ROS) were consistent in these two IAMSLs during anther development and they were potentially associated with the initiation of tapetal PCD. In addition, the activities of ROS-scavenging enzymes increased rapidly, whereas non-enzymatic antioxidants were downregulated together with excess ROS production in IAMSLs. Real-time PCR analysis showed that the expression levels of superoxide dismutase, catalase, and ascorbate peroxidase genes, which encode important antioxidant enzymes, were significantly upregulated during early pollen development. Thus, we inferr...Continue Reading

References

May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Ian M Moller
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·L J SweetloveA H Millar
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Graham Noctor, Christine H. Foyer
Jul 20, 2004·Journal of Experimental Botany·Carlos Guillermo BartoliJuan José Guiamet
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Nov 4, 2004·Biochemical and Biophysical Research Communications·B Srivalli, Renu Khanna-Chopra
Sep 6, 2005·The Plant Cell·Ki-Hong JungGynheung An
Jul 31, 2008·The Plant Cell·Michael K UdvardiWolf-Rüdiger Scheible
Feb 13, 2010·Science·Mark Tester, Peter Langridge
Nov 3, 2010·Functional & Integrative Genomics·Yaou ShenGuangtang Pan
Nov 4, 2010·Journal of Proteome Research·Richard P JacobyNicolas L Taylor
Jun 28, 2012·Frontiers in Oncology·Gianluca Farrugia, Rena Balzan
Aug 28, 2012·Plant Science : an International Journal of Experimental Plant Biology·Shaoqing LiYingguo Zhu
Jul 11, 2013·PloS One·Deepak K RayJonathan A Foley
Oct 24, 2013·Plant & Cell Physiology·María-Teresa SolísPilar S Testillano
Nov 21, 2013·Journal of Experimental Botany·Aaron BaxterNobuhiro Suzuki
Apr 9, 2014·Experimental Gerontology·Beverley M DancyPhilip G Morgan
Apr 24, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Vimal Kumar SemwalRenu Khanna-Chopra
Jul 11, 2014·PloS One·Cristina RubertiMichela Zottini
Nov 9, 2014·Applied Biochemistry and Biotechnology·Jianxin LiQunce Huang
Dec 9, 2014·Genome Génome / Conseil National De Recherches Canada·Wenping GongZujun Yang
Dec 17, 2014·Trends in Cell Biology·Morten Scheibye-KnudsenVilhelm A Bohr
Feb 3, 2015·Nature·A Phillip WestGerald S Shadel
Mar 6, 2015·Frontiers in Plant Science·Veselin PetrovTsanko S Gechev
Oct 27, 2015·Cell·Gerald S Shadel, Tamas L Horvath

❮ Previous
Next ❯

Citations

May 12, 2019·Plant, Cell & Environment·James P Santiago, Thomas D Sharkey
Sep 16, 2020·Plant Molecular Biology·Zaibao ZhangJie Wen
Oct 19, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Cristina Rodríguez-SuárezAntonio Martín
Sep 13, 2020·Journal of Experimental Botany·Ayelén Mariana DistéfanoGabriela Carolina Pagnussat
Jan 5, 2021·Frontiers in Medicine·Zhen-Dong ZhangJian-Yong Li

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission
scanning electron microscopy
PCR
electrophoresis
MDA
light microscopy
AsA

Software Mentioned

cellSens Entry
SPSS
Elements
IPP
Excel Office
NIS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis