Oxidative stress and antioxidant response in Hypericum perforatum L. plants subjected to low temperature treatment

Journal of Plant Physiology
Matúš SkybaEva Cellárová

Abstract

Extreme low temperatures cause plants multiple stresses, among which oxidative stress is presumed to be the major component affecting the resultant recovery rate. Plants of Hypericum perforatum L., which are known especially for the photodynamic activities of hypericins capable of producing reactive oxygen species under exposure to visible light, were observed to display a substantial increase and persistence in active oxygen production at least two months after recovery from cryogenic treatment. In an effort to uncover the causative mechanism, the individual contributions of wounding during explant isolation, dehydration and cold were examined by means of antioxidant profiling. The investigation revealed activation of genes coding for enzymatic antioxidant catalase and superoxide dismutase at both the transcript and protein levels. Interestingly, plants responded more to wounding than to either low-temperature associated stressor, presumably due to tissue damage. Furthermore, superoxide dismutase zymograms showed the Cu/Zn isoforms as the most responsive, directing the ROS production particularly to chloroplasts. Transmission electron microscopy revealed chloroplasts as damaged structures with substantial thylakoid ruptures.

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Citations

Jun 28, 2014·Journal of Plant Physiology·Seyyedeh-Sanam Kazemi-ShahandashtiSeyyedeh-Sanaz Ramezanpour
Jan 17, 2015·Journal of Hazardous Materials·Petr BabulaMarián Hlavna
May 21, 2016·Frontiers in Plant Science·Katarína Bruňáková, Eva Čellárová
Nov 22, 2019·Environmental Science and Pollution Research International·Ze GongCunxin Fan
Nov 19, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Jessica Jeyanthi James AntonySreeramanan Subramaniam

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