Oxidative stress and mitochondrial glutathione in human lymphocytes exposed to clinically relevant anesthetic drug concentrations

Journal of Clinical Anesthesia
G DeloguG Famularo

Abstract

To evaluate the potential of compounds commonly used in anesthesia practice to affect the intracellular oxidant-antioxidant homeostasis of peripheral blood lymphocytes at clinically relevant concentrations; and to study the changes in reactive oxygen species production and measure the mitochondrial glutathione content. Prospective, in vitro study. Experimental medical research laboratory at a University Hospital. Lymphocytes were isolated from the peripheral blood of 15 healthy donors and incubated for 12 hours at 37 degrees C with the following drug concentrations: thiopental sodium 20 mmoL/mL, droperidol 130 micromol/mL, propofol 60 mmoL/mL, and succinylcholine 17 mmoL/mL. Reactive oxygen species (ROS) generation was determined by hydroethidine and 2',7'-dichlorofluorescein diacetate methods. Mitochondrial glutathione level was assessed using monobromobimane staining. Thiopental-treated lymphocytes exhibited an overgeneration of ROS, but no change was detected in mitochondrial glutathione quantity. Propofol and droperidol could not induce any perturbative effect on the oxidative state of T cells, whereas succinylcholine was found to markedly affect lymphocyte oxidative state both by impairing glutathione content and promoting...Continue Reading

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Citations

Sep 26, 2012·Journal of Anesthesia·Muhammad B RafiqueSalman Zubair
Oct 30, 2013·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·James S Green, Ban C H Tsui
Apr 10, 2009·American Journal of Physiology. Cell Physiology·Hiroe ShiratsuchiMarc D Basson
Nov 4, 2010·Critical Care : the Official Journal of the Critical Care Forum·Valeria ManganelliGiovanna Delogu
Jun 24, 2005·PLoS Medicine·Mervyn Singer, Paul Glynne
Sep 29, 2006·Anesthesiology·Stanley Muravchick, Richard J Levy
May 4, 2021·Frontiers in Immunology·Namrita Halder, Girdhari Lal
Mar 14, 2007·Annales Françaises D'anesthèsie Et De Rèanimation·K Nouette-GaulainF Sztark

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