Oxidative stress and protective effect of erythropoietin on methotrexate-induced esophageal damage

Journal of Pediatric Hematology/oncology
Mesut GaripardicErgül Belge Kurutaş

Abstract

An experimental study was conducted to investigate the role of oxidative stress and effects of erythropoietin (EPO) on methotrexate-induced esophageal damage. Twenty-four female Sprague-Dawley rats were equally divided into 3 groups: Sham operation animals (group S) were administered subcutaneous injections of 0.2 mL of 0.9% NaCl; control animals (group MTX) were administered subcutaneous injections of methotrexate (5 mg/kg) and EPO-treated animals (group EPO) were administered subcutaneous injections of methotrexate (5 mg/kg) and EPO (2000 IU/kg) once daily for 4 consecutive days. At the fifth day, the distal 1.5-cm esophageal segments were harvested for biochemical and histologic investigations. Oxidative damage was assessed by measuring the levels of malondialdehyde and nitric oxide, and activities of superoxide dismutase and catalase in homogenized samples of esophageal tissue. Histologic damage to esophageal tissue was scored and total tissue damage scores were calculated. Malondialdehyde levels in the S and EPO groups were significantly lower than those in the MTX group (P<0.05). Catalase and superoxide dismutase activities, and nitric oxide levels in the S and EPO groups were significantly higher than those in the MTX gr...Continue Reading

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Citations

Jan 11, 2016·BMC Complementary and Alternative Medicine·O Jun KwonSeong-Soo Roh
Mar 10, 2018·Current Opinion in Supportive and Palliative Care·Mathias RathePer T Sangild

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