Oxidative stress and rheologic properties of stored red blood cells before and after transfusion to surgical patients

Transfusion
Enika NagababuSteven M Frank

Abstract

The loss of structural and functional integrity of red blood cells (RBCs) during storage, collectively referred to as "storage lesion," has been implicated in reduced oxygen delivery after transfusion. RBCs are highly susceptible to oxidative damage from generation of reactive oxygen species by autoxidation of hemoglobin. Therefore, we examined whether increased oxidative stress (OS) in stored RBCs is associated with impaired cell membrane deformability before or after transfusion. Thirty-four patients undergoing multilevel spine fusion surgery were enrolled. OS in RBCs was assessed by the presence of fluorescent heme degradation products and methemoglobin, which were measured with fluorimetric and spectrophotometric methods, respectively. Deformability and aggregation were determined by ektacytometry in stored RBCs, autologous salvaged RBCs, and posttransfusion blood samples. OS in stored RBCs was significantly increased with longer storage (R = 0.54, p = 0.032) and significantly higher than that in fresh RBCs (9.1 ± 1.3 fluorescent arbitrary units vs. 7.7 ± 0.9 fluorescent arbitrary units, p < 0.001). Deformability decreased (R = -0.60, p = 0.009) with increasing storage duration. OS was elevated (p < 0.05) and deformability ...Continue Reading

References

May 1, 1992·Transfusion·J A KnightH Anstall
Jan 1, 1991·Free Radical Research Communications·J M RifkindP T Manoharan
Apr 11, 1985·Biochimica Et Biophysica Acta·T ShigaM Okazaki
Jan 1, 1994·Free Radical Biology & Medicine·C GiuliviK J Davies
Jul 2, 1998·Biochemical and Biophysical Research Communications·E Nagababu, J M Rifkind
Nov 24, 1999·Free Radical Biology & Medicine·U J DumaswalaK A Sukalski
Feb 12, 2000·American Journal of Surgery·G ZallenC C Silliman
Oct 18, 2000·Free Radical Biology & Medicine·E NagababuJ M Rifkind
Jan 17, 2002·The Journal of Surgical Research·Tamara L BerezinaGeorge W Machiedo
Jun 7, 2002·Archives of Surgery·Patrick J OffnerChristopher C Silliman
Jan 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Melek Bor-KucukatayOguz K Baskurt
Nov 19, 2004·Antioxidants & Redox Signaling·Enika Nagababu, Joseph M Rifkind
May 25, 2007·Critical Care Medicine·Yasser SakrDaniel De Backer
Mar 21, 2008·The New England Journal of Medicine·Colleen Gorman KochEugene H Blackstone
Jun 11, 2008·Journal of Proteomics·Giancarlo LiumbrunoLello Zolla
Dec 5, 2009·Life Sciences·Enika NagababuJoseph M Rifkind
Dec 9, 2009·The FEBS Journal·Tamir Kanias, Jason P Acker
Apr 9, 2010·Nature Medicine·Janet S Lee, Mark T Gladwin
Jun 19, 2010·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·John R Hess
Mar 8, 2012·Transfusion Medicine·B BlasiL Zolla
Sep 13, 2012·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Ralf KargerVolker Kretschmer
Oct 10, 2012·JAMA : the Journal of the American Medical Association·Dean A FergussonStan Shapiro
Mar 2, 2013·Anesthesia and Analgesia·Steven M FrankViachaslau M Barodka
May 24, 2013·International Journal of Molecular Sciences·Anna BogdanovaLars Kaestner
Nov 20, 2013·Blood Cells, Molecules & Diseases·Viachaslau M BarodkaJohn J Strouse
Apr 9, 2015·The New England Journal of Medicine·Jacques LacroixUNKNOWN Canadian Critical Care Trials Group
Apr 9, 2015·The New England Journal of Medicine·Marie E SteinerChristopher P Stowell

❮ Previous
Next ❯

Citations

Sep 9, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Jun ZhangHong Zhou
Jul 3, 2019·Journal of Cellular Physiology·Qiang MengYahan Fan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie
Matthias RedlinAbdulgabar Salama
Cancer Control : Journal of the Moffitt Cancer Center
Lirong Qu, Darrell J Triulzi
© 2021 Meta ULC. All rights reserved