PMID: 26319214Sep 1, 2015Paper

Oxidative stress and ROS metabolism via down-regulation of sirtuin 3 expression in Cmah-null mice affect hearing loss

Aging
Deug-Nam KwonJin-Hoi Kim

Abstract

CMP-Neu5Ac hydroxylase (Cmah) disruption caused several abnormalities and diseases including hearing loss in old age. However, underling molecular mechanisms that give rise to age-related hearing loss (AHL) in Cmah-null mouse are still obscure. In this study, Cmah-null mice showed age-related decline of hearing associated with loss of sensory hair cells, spiral ganglion neurons, and/or stria vascularis degeneration in the cochlea. To identify differential gene expression profiles and pathway associated with AHL, we performed microarray analysis using Illumina MouseRef-8 v2 Expression BeadChip and pathway-focused PCR array in the cochlear tissues of Cmah-null mouse. Pathway and molecular mechanism analysis using differentially expressed genes provided evidences that altered biological pathway due to oxidative damage by low expressed antioxidants and dysregulated reactive oxygen species (ROS) metabolism. Especially, low sirtuin 3 (Sirt3) gene expressions in Cmah-null mice decreased both of downstream regulator (Foxo1 and MnSod) and regulatory transcription factor (Hif1αand Foxo3α) gene expression. Taken together, we suggest that down-regulation of Sirt3 expression leads to oxidative stress and mitochondrial dysfunction by regulat...Continue Reading

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Citations

Apr 9, 2020·International Journal of Molecular Sciences·Ah-Ra LyuYong-Ho Park
Apr 20, 2017·Chembiochem : a European Journal of Chemical Biology·Jonathan Okerblom, Ajit Varki
May 23, 2019·International Journal of Molecular Medicine·Ru-Zhou ZhaoZhi-Bin Yu
Jan 13, 2018·Journal of Cellular Physiology·Margalida Torrens-MasJorge Sastre-Serra
Feb 23, 2020·The International Journal of Neuroscience·Roberta MarozzoArianna Di Stadio
Jul 13, 2019·Cellular & Molecular Biology Letters·Xuan LuoJun-Yi Wang
Sep 11, 2020·Neural Plasticity·Baoyi FengHao Wu
Mar 31, 2016·Cancers·Yuxin ChenWang Min
May 3, 2021·Journal of Clinical Laboratory Analysis·Song YangDengwen Li
May 20, 2021·Journal of Cellular and Molecular Medicine·Jun ZhangQinliang Zhang
Aug 17, 2021·Animal Biotechnology·Hak Myong ChoeXi-Jun Yin

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