Oxidative stress-associated mitochondrial dysfunction in corticosteroid-treated muscle cells

Muscle & Nerve
Yasushi OshimaTakao Mitsui

Abstract

We analyzed the effects of corticosteroid on mitochondrial membrane potentials (DeltaPsi(m)), generation of reactive oxygen species (ROS), and apoptosis in a human rhabdomyosarcoma cell line, RD, and a dopaminergic neuroblastoma cell line, SH-SY5Y. The cell lines were cultured in the presence or absence of dexamethasone and superoxide dismutase (SOD) for up to 1 week. Dexamethasone treatment increased DeltaPsi(m), ROS generation, and apoptosis in proliferating RD cells. Treatment with SOD attenuated ROS generation and apoptosis, but not DeltaPsi(m). The increase in DeltaPsi(m) seemed to be the primary effect of dexamethasone on proliferating RD cells, which is probably mediated by mitochondrial transcription. In differentiated RD cells, but not differentiated SH-SY5Y cells, dexamethasone treatment showed a delayed effect of interfering with the DeltaPsi(m) and increasing ROS generation and apoptosis. Since these changes disappeared in the presence of SOD, dexamethasone primarily induced ROS generation, resulting in apoptosis. We speculate that this mechanism provides the basis of a pathophysiological model of corticosteroid myopathy.

References

Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Sep 1, 1992·Mutation Research·C Richter
Aug 1, 1991·Italian Journal of Neurological Sciences·M MaroldaA Gentile
Sep 16, 1991·Biochemical and Biophysical Research Communications·M HayakawaT Ozawa
Oct 20, 1995·The Journal of Biological Chemistry·S M Tanhauser, P J Laipis
Dec 15, 1994·International Journal of Cancer. Journal International Du Cancer·A BravardC Luccioni
Feb 1, 1993·Neurology·H J Tritschler, R Medori
Jul 1, 1996·Journal of the Neurological Sciences·T MitsuiS Saito
Sep 26, 1997·Nature·K PolyakB Vogelstein
Oct 10, 2001·Journal of Neuroscience Research·C R MurthyM D Norenberg
Jan 26, 2002·Acta Neuropathologica·Yoshifumi UmakiToshio Matsumoto
Aug 27, 2002·Journal of Neurology·Takao MitsuiToshio Matsumoto

❮ Previous
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Citations

Jun 8, 2007·Cellular and Molecular Neurobiology·Gregg R WardAllyn C Howlett
Apr 2, 2011·Pathology Oncology Research : POR·Judit DobosAndrea Ladányi
Jan 1, 2011·Polish Journal of Veterinary Sciences·I Otrocka-Domagała
Oct 21, 2009·Drug and Chemical Toxicology·Josef Finsterer, Liane Segall
Mar 30, 2011·Rheumatic Diseases Clinics of North America·Adam MorMichael H Pillinger
Jun 13, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Amie J Dirks-Naylor, Carrie L Griffiths
May 15, 2007·Trends in Endocrinology and Metabolism : TEM·Irini ManoliGeorge P Chrousos
Feb 15, 2008·Australian Veterinary Journal·S E DaviesW J Bruce
Feb 3, 2007·Muscle & Nerve·Jennifer S Moylan, Michael B Reid
Aug 15, 2006·Biochemical and Biophysical Research Communications·Yukiko KurodaToshio Matsumoto
Jan 16, 2016·Chronic Respiratory Disease·Marios PanagiotouCharlie Strange
Jan 1, 2013·Cells·Amanda L Gruver-Yates, John A Cidlowski
May 27, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sachchida Nand PandeyYi-Wen Chen
Feb 21, 2018·QJM : Monthly Journal of the Association of Physicians·M HaranL Shvidel
Oct 22, 2010·Current Opinion in Neurology·Patrick RothMichael Weller
May 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lorenzo Di Cesare MannelliCarla Ghelardini
Jun 11, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irini ManoliMarc R Blackman
Sep 3, 2014·Reviews in the Neurosciences·Alberto MachadoJosefina Cano
Jun 12, 2020·International Journal of Molecular Sciences·Elisa BalboaJuan C Sáez
Jun 30, 2018·Oxidative Medicine and Cellular Longevity·Mateusz Jakub KarniaJan Jacek Kaczor
Feb 13, 2019·BMC Veterinary Research·Iwona Otrocka-DomagałaMichał Gesek
Jan 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hyunjung LeeJiyun Ahn
Apr 14, 2021·Archives of Biochemistry and Biophysics·Anayt UllaTakeshi Nikawa

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis