Oxidative stress at different stages of two-step semen cryopreservation procedures in dogs

Theriogenology
C F LucioC I Vannucchi

Abstract

Sperm cryopreservation generates sperm damage and reduced fertilization capacity as a consequence of reactive oxygen species formation. Identifying the critical points of the process will contribute to the development of strategies for oxidative stress prevention. Therefore, the aim of this experiment was to verify the occurrence of oxidative stress during the two-step cryopreservation process in dogs. Six healthy mature dogs were used and submitted to the two-step sperm cryopreservation protocol. The sperm analysis was done at three time points: after refrigeration, after glycerolization, and after thawing by sperm motility, measurement of spontaneous and induced oxidative stress, sperm mitochondrial activity, plasma membrane integrity, flow cytometric evaluation of plasma and acrosome membrane integrity, mitochondrial membrane potential, and sperm chromatin structure assay. There was an increase in free radical production after glycerolization (87.4 ± 15.5 ng/mL of spontaneous thiobarbituric acid reactive substances (TBARS) after refrigeration and 1226.3 ± 256.0 ng/mL after glycerolization; P < 0.05), in association with loss of sperm mitochondrial activity. However, frozen-thawed samples had lower sperm motility, lower resis...Continue Reading

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Citations

Apr 8, 2017·Animal Reproduction Science·D S R AngrimaniC I Vannucchi
Jun 29, 2017·Animal Biotechnology·Andressa DalmazzoValquíria H Barnabe
Mar 30, 2019·Acta Veterinaria Hungarica·Zhao NamulaTakeshige Otoi
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Aug 21, 2020·Biopreservation and Biobanking·Nazan KeskinSukru Dursun
Feb 26, 2019·Journal of Assisted Reproduction and Genetics·Eva TvrdáJaime Gosálvez
Jul 23, 2020·Basic and Clinical Andrology·Daniel S R AngrimaniCamila I Vannucchi
Mar 14, 2020·Theriogenology·Gaia Cecilia Luvoni, Martina Colombo
Dec 16, 2019·Theriogenology·Eva Patricia López-DamiánTatiana Fiordelisio
Jul 6, 2021·Veterinary World·Ghadeer Sabah Bustani, Falah Hasan Baiee
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Feriel Yasmine Mahiddine, Min-Jung Kim
Apr 27, 2021·The Journal of Veterinary Medical Science·Seonggyu BangJongki Cho
Aug 7, 2021·Antioxidants·Eliana Pintus, José Luis Ros-Santaella
Sep 14, 2021·Biopreservation and Biobanking·Michelle Silva AraujoFabiana Ferreira de Souza
Sep 30, 2021·Reproduction, Fertility, and Development·Brahim Halil GüngörGaffari Türk
Nov 9, 2021·Reproduction in Domestic Animals = Zuchthygiene·Maria F GallelliNorma Monachesi

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