Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

Arthritis Research & Therapy
Emese BaloghMonika Biniecka

Abstract

In this study, we examined the effect of oxidative stress on cellular energy metabolism and pro-angiogenic/pro-inflammatory mechanisms of primary rheumatoid arthritis synovial fibroblast cells (RASFC) and human umbilical vein endothelial cells (HUVEC). Primary RASFC and HUVEC were cultured with the oxidative stress inducer 4-hydroxy-2-nonenal (4-HNE), and extracellular acidification rate, oxygen consumption rate, mitochondrial function and pro-angiogenic/pro-inflammatory mechanisms were assessed using the Seahorse analyser, complex I-V activity assays, random mutation mitochondrial capture assays, enzyme-linked immunosorbent assays and functional assays, including angiogenic tube formation, migration and invasion. Expression of angiogenic growth factors in synovial tissue (ST) was assessed by IHC in patients with rheumatoid arthritis (RA) undergoing arthroscopy before and after administration of tumour necrosis factor inhibitors (TNFi). In RASFC and HUVEC, 4-HNE-induced oxidative stress reprogrammed energy metabolism by inhibiting mitochondrial basal, maximal and adenosine triphosphate-linked respiration and reserve capacity, coupled with the reduced enzymatic activity of oxidative phosphorylation complexes III and IV. In contr...Continue Reading

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Citations

Oct 26, 2018·Clinical and Experimental Immunology·U FearonJ M Fletcher
Jan 17, 2019·Annals of the New York Academy of Sciences·Natalia S HarasymowiczFarshid Guilak
Mar 3, 2019·Nature Reviews. Rheumatology·Oore-Ofe Olumuyiwa-AkeredoluEtheresia Pretorius
May 14, 2020·International Journal of Molecular Sciences·Nuria Garrido-PérezEduardo Ruiz-Pesini
Jul 3, 2020·Clinical Rheumatology·Muzhe LiZhiwei Chen
Jan 24, 2021·Arthritis Research & Therapy·Cuiyan WuFeng Zhang
Mar 16, 2021·Frontiers in Immunology·Weiji LinShenghao Tu
Mar 19, 2019·Drug Discovery Today·M Pilar Bayona-BafaluyEduardo Ruiz-Pesini
May 15, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dan-Na HuangXi-Jun Wang
Jun 1, 2021·Immunopharmacology and Immunotoxicology·Payam Hashemi, Shaghayegh Pezeshki
May 18, 2021·Free Radical Biology & Medicine·Julia Bahja, Marcus K Dymond
Sep 21, 2021·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Long WangTao Luo
Sep 21, 2021·Frontiers in Cardiovascular Medicine·Bowen LiYuanming Wu

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Methods Mentioned

BETA
biopsies
FCS
Assay
enzyme-linked immunosorbent assays
ELISA
random mutation capture

Software Mentioned

AnalySIS
SoftMax Pro
SPSS Statistics

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