Oxidative stress-induced Akt downregulation mediates green tea toxicity towards prostate cancer cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Anna Maria PosadinoGianfranco Pintus

Abstract

Green tea consumption has been shown to possess cancer chemopreventive activity. Polyphenol E (PE) is a widely used standardized green tea extract formulation. This study was designed to investigate the impact of PE on prostate cancer cells (PC3), analyze the potential signals involved and elucidate whether anti- or pro-oxidant effects may be implicated. Treatment of PC3 cells with 30 and 100μg/ml PE significantly decreased cell viability and proliferation. At the tested concentrations, PE did not exert any antioxidant activity, eliciting instead a pro-oxidant effect at concentrations 30 and 100μg/ml, which was consistent with the observed PE cytotoxicity. PE-induced cell death was associated with mitochondrial dysfunction and downregulation of Akt activation, thus suggesting their implication in the PE-elicited cell dysfunction. Cell exposure to the ROS scavenger N-Acetyl Cysteine prevented PE-induced ROS increase, pAkt impairment, and cell death, clearly indicating the causative role of ROS in the observed phenomena. Failure of PE to induce PC3 damage in cells overexpressing Akt further confirms its implication in the PE-elicited cell death. Our findings showed an association between the antiproliferative and the pro-oxidant ...Continue Reading

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Citations

Aug 4, 2018·Journal of Applied Toxicology : JAT·Meran Keshawa EdiriweeraSameera Ranganath Samarakoon
Mar 15, 2019·Critical Reviews in Food Science and Nutrition·Xiao-Yu XuHua-Bin Li
Nov 19, 2019·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Alexander ReisingerGerald Hackl
Aug 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Maria PosadinoGianfranco Pintus
Jun 30, 2018·Nutrients·Sajid Ur RahmanXichun Wang
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mudhi AlAliSwee-Hua Erin Lim
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberta GiordoGianfranco Pintus

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