Oxidative stress-induced apoptotic insults to rat osteoblasts are attenuated by nitric oxide pretreatment via GATA-5-involved regulation of Bcl-X L gene expression and protein translocation

Archives of Toxicology
Gong-Jhe WuRuei-Ming Chen

Abstract

Nitric oxide (NO) has biphasic effects on regulating osteoblast survival and death. This study was aimed to evaluate the effects of NO pretreatment on hydrogen peroxide (HP)-induced insults of rat osteoblasts and the possible mechanisms. Exposure of osteoblasts prepared from rat calvarias to HP significantly increased intracellular reactive oxygen species levels, decreased alkaline phosphatase activity and cell survival, and ultimately induced cell apoptosis. However, NO pretreatment lowered HP-induced oxidative stress and apoptotic insults. In parallel, HP increased Bax levels and its translocation from the cytoplasm to mitochondria. NO pretreatment caused significant attenuations in HP-induced modulations in Bax synthesis and translocation. In contrast, pretreatment with NO enhanced levels and translocation of antiapoptotic Bcl-XL protein in rat osteoblasts. RNA analyses further revealed that HP inhibited Bcl-XL mRNA expression without affecting Bax mRNA levels. In comparison, NO induced Bcl-XL mRNA production and alleviated HP-caused inhibition of this mRNA expression. As to the mechanism, HP suppressed RNA and protein levels of transcription factor GATA-5 in rat osteoblasts. Pretreatment with NO induced GATA-5 mRNA and prot...Continue Reading

References

Mar 1, 1995·Journal of Cellular Biochemistry·P Collin-OsdobyP Osdoby
Mar 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D M Evans, S H Ralston
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y T HsuR J Youle
Aug 1, 1997·British Journal of Rheumatology·S H Ralston
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C H CottartJ P Clot
Sep 29, 1999·Mammalian Genome : Official Journal of the International Mammalian Genome Society·G NemerM Nemer
Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K E ArmourS H Ralston
Nov 25, 2000·European Journal of Pharmacology·C E FioreG Clementi
May 23, 2001·Journal of Bone and Mineral Metabolism·S Takeda, G Karsenty
Nov 6, 2001·Advances in Nephrology From the Necker Hospital·G Karsenty
Mar 29, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ruei-Ming ChenJyh-Horng Wang
Mar 20, 2003·The Journal of Biological Chemistry·Makoto Kawatani, Masaya Imoto
Sep 25, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·An Van LaethemPatrizia Agostinis
Jan 22, 2005·Seminars in Cell & Developmental Biology·John B E Burch
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ruei-Ming ChenChia-Chen Chang
Jun 21, 2005·Annals of the New York Academy of Sciences·Wei-Pin HoRuei-Ming Chen
Jun 22, 2005·The Journal of Biological Chemistry·Yung-Luen YuJeffrey J Y Yen
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lisa J PagliariDouglas R Green
Aug 19, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chia-Chen ChangRuei-Ming Chen
Apr 26, 2008·Endocrine Reviews·Andrea GiustinaErnesto Canalis
May 26, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ruei-Ming ChenChih-Wei Chou
Oct 14, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Huai-Chia ChangRuei-Ming Chen
Feb 1, 2011·Nitric Oxide : Biology and Chemistry·Daniel MurilloSruti Shiva
Aug 9, 2012·Antioxidants & Redox Signaling·Clement CochainJean-Sébastien Silvestre
Sep 8, 2012·Cellular and Molecular Life Sciences : CMLS·Giovanni MonacoGeert Bultynck
Jan 12, 2013·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S A JamalC J Hamilton
Mar 19, 2014·Journal of Molecular and Cellular Cardiology·Jillian N SimonRicardo Carnicer
May 31, 2014·Archives of Biochemistry and Biophysics·Natalie A Sims, Christina Vrahnas

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Citations

Nov 25, 2017·Experimental & Molecular Medicine·Mei-Hsiu LiaoRuei-Ming Chen
Oct 19, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroshi KanekoRitsuko Shimizu
May 17, 2019·The American Journal of Chinese Medicine·Ming-Chung LinRuei-Ming Chen
Mar 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gong-Jhe WuRuei-Ming Chen
Feb 16, 2017·International Journal of Oncology·Bor-Chin ChengRuei-Ming Chen
Aug 14, 2020·Neural Regeneration Research·Han-A ParkElizabeth A Jonas

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