Oxidative stress induced by Cremophor EL is not accompanied by changes in NF-kappaB activation or iNOS expression

Toxicology
María Belén GutiérrezMaría J Tuñón

Abstract

The effects of polyoxyethylenglycerol triricinoleate 35 (Cremophor EL, CrEL) on markers of oxidative stress, nuclear factor kappa B (NF-kappaB) activation and inducible nitric oxide synthase (iNOS) expression were studied in the liver of male Wistar rats. Animals were randomly divided into three groups. Group Cr1 received, i.p., CrEL at 0.046ml/kg daily for 7 days, group Cr2 received CrEL at 0.33ml/kg and the controls were injected with CrEL vehicle (saline solution with 25% ethanol). Both alanine transaminase (ALT) and aspartate transaminase (AST) serum activities were significantly increased in the Cr2 group (+16% and +25%, respectively). AST activity was also higher in the Cr1 group when compared to control animals (+20%). The cytosolic concentration of thiobarbituric acid reactive substances (TBARS) increased in both groups of rats receiving CrEL (Cr1: +24%; Cr2: +33%). Reduced glutathione (GSH) concentration was not significantly modified at any of the CrEL doses, but both the hepatic concentration of oxidised glutathione (GSSG) (Cr1: +37%; Cr2: +84%) and the GSH/GSSG ratio (Cr1: -21%; Cr2: -45%) were significantly modified. CrEL induced no significant NF-kappaB activation, changes in p50 and p65 NF-kappaB subunits or indu...Continue Reading

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Citations

Dec 5, 2012·Comparative Hepatology·Nelson Alexandre KretzmannClaudio Augusto Marroni
Jul 20, 2012·Journal of Biomedicine & Biotechnology·Graziella Ramos RodriguesNorma Possa Marroni
Feb 1, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Irene CrespoJavier González-Gallego
Mar 25, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Fernanda C CamposRubens Cecchini
Aug 30, 2014·World Journal of Gastroenterology : WJG·Gilmara Pandolfo ZabotHenrique Sarubbi Fillmann
Mar 19, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Renata GrespanRoberto Kenji Nakamura Cuman

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