Oxidative stress-induced Gadd45α inhibits trophoblast invasion and increases sFlt1/sEng secretions via p38 MAPK involving in the pathology of pre-eclampsia

The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
Xiru LiuHongbo Qi

Abstract

Pre-eclampsia (PE) is one of the most common pregnancy-related complications. We have previously reported that growth arrest and DNA damage-inducible 45 alpha (Gadd45α) is over-expressed in trophoblasts in pre-eclamptic placentas, with an excessive activation of p38 mitogen-activated protein kinase (MAPK) and increased levels of soluble Fms-like tyrosine kinase 1 (sFlt-1) and soluble endoglin (sEng) in maternal sera. Now we further investigate how Gadd45α regulates trophoblast functions and anti-angiogenesis factors secretions during placental development in patients with PE. Human placental villous explants were used to verify the effects of Gadd45α and p38 MAPK in placentation. Then HRT8/SVneo cells exposed to hypoxia/reoxygenation (H/R) were employed as an oxidative stress model to investigate the effects of Gadd45α on invasion and sFlt-1/sEng secretions. Through silencing Gadd45α with lentiviral vector-based short-hairpin RNA and inhibiting p38 MAPK with SB203580, we demonstrated that Gadd45α and its downstream p38 protein played roles in the pathology of pre-eclampsia. Gadd45α was found to have increased expression in H/R-treated villous explants and HTR8/SVneo cells. Gadd45α knockdown or p38 blockage could promote trophob...Continue Reading

References

Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A J FornaceM C Hollander
Feb 18, 1999·Molecular and Cellular Biology·F CarrierA J Fornace
Feb 7, 2004·The Journal of Clinical Endocrinology and Metabolism·Ravi ThadhaniS Ananth Karumanchi
Feb 7, 2004·The New England Journal of Medicine·Richard J LevineS Ananth Karumanchi
Aug 6, 2004·Reproductive Biology and Endocrinology : RB&E·Elsebeth Staun-RamEliezer Shalev
Sep 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Luiz F ZerbiniJin-Rhong Zhou
Oct 19, 2004·Endocrinology·S Ananth Karumanchi, Yuval Bdolah
Jun 11, 2005·Science·Christopher W Redman, Ian L Sargent
Aug 2, 2006·Obstetrics and Gynecology·Caroline SignoreRichard J Levine
Dec 19, 2006·Taiwanese Journal of Obstetrics & Gynecology·Tai-Ho Hung, Graham J Burton
May 24, 2008·The International Journal of Biochemistry & Cell Biology·Irmgard Irminger-FingerOlivier Irion
Dec 17, 2008·Placenta·J M Roberts, C A Hubel
Jan 14, 2009·Placenta·C W G Redman, I L Sargent
Aug 20, 2009·Current Protein & Peptide Science·Ming GaoLun Song
Dec 20, 2011·Lancet·Andrew H ShennanFiona Milne
Jan 10, 2012·Disease Models & Mechanisms·Kathleen A PenningtonDanny J Schust
Apr 21, 2012·Current Molecular Medicine·R E TamuraL F Zerbini
Jun 22, 2012·Journal of Cellular Physiology·Yali XiongOssie Geifman-Holtzman
Jul 25, 2012·Cancer Biology & Therapy·Zhanhai ShanDan Li
Sep 5, 2012·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·S MasouraN Vavatsi
Jan 19, 2013·The Journal of Biological Chemistry·Fang YangQimin Zhan
May 28, 2013·Journal of Translational Medicine·Jennifer S TrontDan A Liebermann
Nov 21, 2013·Placenta·A L GregoryM Letarte

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Citations

Jun 22, 2016·Seminars in Immunopathology·Lars BremerGisa Tiegs
Jan 25, 2017·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Roberto RomeroTinnakorn Chaiworapongsa
Jan 17, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Colette N MillerJanice A Dye
Jul 1, 2020·Biomolecules·Manoj Kumar JenaNihar Ranjan Nayak
Dec 28, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Delia I ChiarelloReinaldo Marín
Nov 19, 2020·International Journal of Molecular Sciences·Jin SeokGi Jin Kim
Apr 4, 2021·Antioxidants·Xiang-Qun Hu, Lubo Zhang
Jun 23, 2021·Journal of the American Society of Nephrology : JASN·Theresa M WewersGiovana S Di Marco
Aug 17, 2021·Computational and Mathematical Methods in Medicine·Dandan QuanManzhen Zuo
Nov 17, 2021·Molecular and Cellular Biochemistry·Georgia Margioula-SiarkouGeorgios Mavromatidis

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