Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells

Molecular Human Reproduction
E TskitishviliK Shimoya

Abstract

Oxidative stress with elevated intracellular Ca(2+) concentration as well as endothelial dysfunction is a component of pre-eclampsia. Our aim was to investigate the oxidative stress-dependent expression of Endoglin and Ca(2+)-binding S100B protein from villous and amniotic tissue cultures, and to assess sEng expression from S100B protein-stimulated endothelial cells. We initially examined Endoglin and Hydroxy-nonenal-(HNE)-modified proteins in the placentas and amnion obtained from women with pre-eclampsia (n = 8), and healthy controls (n = 8) by immunohistochemistry. To examine oxidative stress and the S100B protein effect on sEng expression from endothelial cells, normal villous and amniotic tissue cultures were stimulated by 4-HNE, sodium fluoride and xanthine/xanthine oxidase, whereas human umbilical vein endothelial cell cultures were treated with S100B protein in a dose- and time-dependent manner at 37 degrees C in an environment of 95% air and 5% of CO(2). Culture supernatants were assessed using ELISA. Cell viability was determined using MTS assay. The concentrations of sEng and S100B protein were significantly increased in the villous and amniotic tissue culture supernatants under oxidative stress. S100B protein-stimul...Continue Reading

References

Feb 1, 1993·Experimental Cell Research·M S PepperR Montesano
Jun 1, 1996·The Journal of Cell Biology·P LastresC Bernabéu
Nov 24, 1999·The Journal of Clinical Investigation·A BourdeauM Letarte
Jul 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S W WalshL J Roberts
Dec 18, 2001·Mechanisms of Development·Leon Jonker, Helen M Arthur
Apr 3, 2002·The EMBO Journal·Marie-José GoumansPeter ten Dijke
Jul 13, 2002·Journal of Cellular Physiology·Jennifer L Martindale, Nikki J Holbrook
May 15, 2003·Journal of Cell Science·Chenggang LiShant Kumar
Aug 12, 2003·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Christy-Lynn M CookeSandra T Davidge
Aug 30, 2003·Stroke; a Journal of Cerebral Circulation·Yonghua ZhuDavid A Greenberg
Oct 24, 2003·Virchows Archiv : an International Journal of Pathology·Yasushi TakagiIkuo Konishi
Jan 25, 2005·Cardiovascular Research·Franck LebrinPeter Ten Dijke
Oct 19, 2005·Hypertension·James M Roberts, Hilary S Gammill
Jun 6, 2006·Nature Medicine·Shivalingappa VenkateshaS Ananth Karumanchi
Sep 8, 2006·The New England Journal of Medicine·Richard J LevineUNKNOWN CPEP Study Group
Dec 19, 2006·Taiwanese Journal of Obstetrics & Gynecology·Tai-Ho Hung, Graham J Burton
May 11, 2007·Journal of Reproductive Immunology·Hernan D Kopcow, S Ananth Karumanchi
Aug 11, 2007·American Journal of Obstetrics and Gynecology·Christopher J Robinson, Donna D Johnson
Aug 11, 2007·American Journal of Obstetrics and Gynecology·Anne Cathrine StaffNina Kittelsen Harsem
Jul 25, 2008·Nature·Kezhong Zhang, Randal J Kaufman
Jul 26, 2008·The American Journal of Pathology·Christopher W G Redman
Oct 31, 2008·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Chikako HirashimaMitsuaki Suzuki
Jan 19, 2010·British Journal of Nursing : BJN·Sarah Lewis

❮ Previous
Next ❯

Citations

Mar 8, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Toshiyuki SadoHiroshi Kobayashi
Apr 1, 2012·Pregnancy Hypertension·J VettorazziJ G Ramos
Jan 13, 2015·Archives of Gynecology and Obstetrics·Burcu Artunc-UlkumenCeyhun Gozukara
Mar 13, 2015·Frontiers in Genetics·Mirjana Jerkic, Michelle Letarte
May 2, 2013·Journal of Leukocyte Biology·Estibalitz Laresgoiti-Servitje
May 13, 2019·Molecular and Cellular Biochemistry·Kailash Prasad
May 24, 2013·Analytical Chemistry·Maogen ZhangWaldemar Gorski
Oct 31, 2021·Journal of Perinatal Medicine·Mandy BusseAna Claudia Zenclussen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Reproductive Biology and Endocrinology : RB&E
Simon GrillOlav Lapaire
Hypertension
James M RobertsNHLBI Working Group on Research on Hypertension During Pregnancy
Clinica Chimica Acta; International Journal of Clinical Chemistry
Cristina NettoRoberto Giugliani
© 2022 Meta ULC. All rights reserved