PMID: 9648855Jul 2, 1998Paper

Oxidative stress induces a form of programmed cell death with characteristics of both apoptosis and necrosis in neuronal cells

Journal of Neurochemistry
S TanP Maher

Abstract

Oxidative stress is implicated in a number of neurological disorders including stroke, Parkinson's disease, and Alzheimer's disease. To study the effects of oxidative stress on neuronal cells, we have used an immortalized mouse hippocampal cell line (HT-22) that is particularly sensitive to glutamate. In these cells, glutamate competes for cystine uptake, leading to a reduction in glutathione and, ultimately, cell death. As it has been reported that protein kinase C activation inhibits glutamate toxicity in these cells and is also associated with the inhibition of apoptosis in other cell types, we asked if glutamate toxicity was via apoptosis. Morphologically, glutamate-treated cells underwent plasma membrane blebbing and cell shrinkage, but no DNA fragmentation was observed. At the ultrastructural level, there was damage to mitochondria and other organelles although the nuclei remained intact. Protein and RNA synthesis inhibitors as well as certain protease inhibitors protected the cells from glutamate toxicity. Both the macromolecular synthesis inhibitors and the protease inhibitors had to be added relatively soon after the addition of glutamate, suggesting that protein synthesis and protease activation are early and distinct...Continue Reading

Citations

Jun 15, 2000·Journal of Neurochemistry·K ChenM D Maines
Jan 9, 2001·Journal of Neurochemistry·R DarguschD Schubert
Mar 10, 2001·The Journal of Cell Biology·S TanD Schubert
Oct 12, 2001·Journal of Neurochemistry·R IentileS Macaione
Jul 18, 2002·The Journal of Biological Chemistry·Yutaka SagaraPamela Maher
Sep 28, 2002·The International Journal of Neuroscience·Débora C HipólideJosé N Nobrega
Oct 14, 2004·Experimental Brain Research·Ferenc GallyasMária Mázló
Jul 28, 2005·Developmental Neuroscience·Claire W McLeanDonna M Ferriero
Sep 27, 2007·CNS Drug Reviews·Pierre Etienne Chabrier, Michel Auguet
Mar 14, 2007·The Journal of Gene Medicine·A L C CardosoM C Pedroso de Lima
Jun 6, 2009·Cellular and Molecular Neurobiology·Yuen-Shan HoRaymond Chuen-Chung Chang
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Sergio TufikMarco T de Mello
Jun 8, 2012·Journal of Neuroendocrinology·F L CamposG Baltazar
Oct 19, 2013·Bulletin of Experimental Biology and Medicine·I O LogvinovS B Seredenin
Jun 30, 2015·The American Journal of Chinese Medicine·Sung Min AhnByung Tae Choi
Apr 7, 2016·Cellular and Molecular Life Sciences : CMLS·Jennifer Yinuo Cao, Scott J Dixon
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chang-Hyun ParkHyung-Ho Lim
Apr 17, 2020·Frontiers in Neuroscience·Vignayanandam Ravindernath MuddapuM Michael Gromiha
Mar 10, 2001·Journal of Neurochemistry·P J Kingham, J M Pocock
Dec 18, 2001·Journal of Applied Physiology·Shao-Hua YangJames W Simpkins
Mar 11, 2003·Antioxidants & Redox Signaling·Krista L SeanorDennis J Templeton
Jan 10, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joseph BurdoDavid Schubert
Nov 23, 2006·Antioxidants & Redox Signaling·Stefan W RyterAugustine M K Choi
Oct 14, 2003·The Journal of Comparative Neurology·Elizabeth Jane FryNorman Ruthven Saunders
Dec 6, 2006·Proteomics·Youra LeeDo Hee Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis