Oxidative stress induces senescence and sterile inflammation in murine amniotic cavity

Placenta
Jossimara PolettiniRamkumar Menon

Abstract

A physiologic increase of reactive oxygen species (ROS) is observed through pregnancy. ROS-induced damage to major cellular elements, specifically protein peroxidation, can lead to fetal and placental tissue senescence and inflammation often associated with normal parturition. The purpose of this study was to examine the effects of oxidative stress (OS) in inducing changes in proteins, senescence, and sterile inflammation in pregnant mice. CD-1 mice (n = 5/group) on day 14 of gestation were subjected to minilaparotomy and the uterine horn between gestational sacs was injected with the following: saline (control), cigarette smoke extract (CSE) CSE diluted in saline and CSE + SB 203580 (SB) (a p38 mitogen-activated protein kinase (MAPK) inhibitor). Mice were sacrificed on day 18, and amniotic sacs, placentas and amniotic fluid (AF) were collected. Protein damage was evaluated by immunostaining for 3-Nitrotyrosine modified proteins (3-NT). Activation of prosenescence p38MAPK was evaluated by western blot. Senescence features, β-galactosidase (SA-β-Gal) and AF inflammatory cytokines were analyzed by immunostaining and multiplex luminex-based immunoassays, respectively. The data were analyzed by ANOVA and Tukey's test, p < .05 was u...Continue Reading

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Citations

Mar 9, 2019·Biology of Reproduction·Damian D Guerra, K Joseph Hurt
May 1, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lauren RichardsonRamkumar Menon
Feb 13, 2020·Reproductive Sciences·Brunna Nepomuceno ColattoJossimara Polettini
Dec 10, 2019·American Journal of Reproductive Immunology : AJRI·Narmada LavuRamkumar Menon
Feb 13, 2020·Science Signaling·Lauren S RichardsonRamkumar Menon
Sep 27, 2020·Placenta·Ramkumar MenonRheanna Urrabaz-Garza
Oct 31, 2020·Frontiers in Physiology·Jossimara Polettini, Marcia Guimarães da Silva
Oct 9, 2021·Cell Communication and Signaling : CCS·Megan C ShepherdRamkumar Menon
Dec 3, 2021·PloS One·Nicholas R SpencerRamkumar Menon

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