Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production: stress biomarker or oxidative stress signalling molecule?

Toxicology in Vitro : an International Journal Published in Association with BIBRA
L FoucaudJ Falla

Abstract

The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of 4-HNE in inducing further cytotoxic effects. ROS production in cells by CB nanoparticles was shown by the oxidation of DCFH after a short time exposure. These particles induced the formation of 4-HNE-protein adducts and significant modification of glutathione content corresponding to an increase of oxidized glutathione form (GSSG) and a decrease of total glutathione (GSX) content. These results attest to an oxidative stress induced by the carbon black nanoparticles, although no induction of HO-1 protein expression was detected. Concerning the effects of a direct exposure to 4-HNE, our results showed that 4-HNE is not cytotoxic for concentrations lower than 12.5 microM. By contrast, it provokes a very high cytotoxicity for concentrations above 25 microM. An induction of HO-1 expression was observed from concentrations above 5 microM of 4-HNE. Finally, glutathione content decreased significantly from 5 microM of 4-HNE but no modification was observed under this co...Continue Reading

References

Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
Nov 1, 1993·American Journal of Respiratory Cell and Molecular Biology·M E HazbunW L Eschenbacher
Oct 1, 1993·Scandinavian Journal of Immunology·R HuangL Hellman
Aug 1, 1996·American Journal of Respiratory Cell and Molecular Biology·R F HamiltonA Holian
Dec 1, 1996·Toxicology and Applied Pharmacology·A KirichenkoA Holian
Jun 6, 1998·The Journal of Biological Chemistry·S SrivastavaA Bhatnagar
Jan 5, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·A M CasillasA E Nel
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·N E AvissarJ N Finkelstein
May 24, 2001·Archives of Biochemistry and Biophysics·S W Luckey, D R Petersen
Feb 14, 2002·Free Radical Biology & Medicine·Jonathan P CoeDavid J Harrison
Dec 31, 2002·Journal of Immunological Methods·Branislava JanicJoanna Floros
Apr 12, 2003·Progress in Lipid Research·Koji Uchida
Aug 2, 2003·Molecular Aspects of Medicine·R J Schaur
Aug 2, 2003·Molecular Aspects of Medicine·Mario Umberto Dianzani
Oct 14, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·D M BrownV Stone
Aug 4, 2004·Free Radical Biology & Medicine·Henry Jay Forman, Dale A Dickinson
Jan 15, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Deborah KlestadtJaïro Falla
Mar 23, 2005·Free Radical Biology & Medicine·Ingrid Beck-SpeierJoachim Heyder
Oct 26, 2005·Particle and Fibre Toxicology·Ken DonaldsonVicki Stone
Jun 13, 2006·Chemosphere·Eiko Koike, Takahiro Kobayashi
Aug 2, 2006·Journal of the Air & Waste Management Association·Andrés AlastueySaul García dos Santos
Aug 24, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C E L PereiraC R Rhoden
Dec 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V StoneK Donaldson

❮ Previous
Next ❯

Citations

Jun 1, 2011·Analytical and Bioanalytical Chemistry·Silvia DiabatéCarsten Weiss
Dec 9, 2015·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·James ClarkBastiaan Hoogendoorn
Jul 20, 2011·Journal of Proteomics·Daniela BraconiAnnalisa Santucci
Jul 4, 2012·The Science of the Total Environment·Adny Henrique SilvaTânia Beatiz Creczynski-Pasa
Sep 18, 2014·Animal : an International Journal of Animal Bioscience·H HassanpourA Mohebbi
Jun 14, 2017·ACS Applied Materials & Interfaces·Cheng-Chung ChouChung-Yuan Mou
Dec 18, 2013·Annual Review of Public Health·Douglas S Diekema
Mar 7, 2017·Turkish Journal of Medical Sciences·Zerrin Defne DündarBaşar Cander
Aug 24, 2017·Reproduction in Domestic Animals = Zuchthygiene·P Martín MuñozC Ortega Ferrusola

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

© 2021 Meta ULC. All rights reserved