Oxidative stress is a triggering factor for LPS-induced Mrp2 internalization in the cryopreserved rat and human liver slices

Biochemical and Biophysical Research Communications
Shuichi SekineToshiharu Horie

Abstract

Cholestasis develops during inflammation and is characterized as occurring under oxidative stress. We have described the internalization of multidrug resistance-associated protein 2 (Mrp2), a biliary transporter involved in bile-salt-independent bile flow, under ethacrynic acid or lipopolysaccharide (LPS)-induced acute oxidative stress in rat liver. However, it remains unclear whether canalicular Mrp2 internalization is observed in human liver under conditions of acute oxidative stress. In this study, we examined the effect of dimerumic acid (DMA), an antioxidant and found in traditional Chinese medicine, on endotoxin-induced Mrp2 internalization in rat and human liver slices. At 1.5h following LPS treatment (100microg/mL), canalicular Mrp2 localization was disrupted without changing the expression of Mrp2 protein or the integrity of filamentous actin in the rat and human liver slices. Pretreatment with DMA (10microM) counteracted LPS-induced subcellular distribution of Mrp2. Our data clearly indicated that LPS-induced short-term rapid retrieval of Mrp2 from the canalicular surface resulted from LPS-induced oxidative stress in rat and human liver slices.

References

Dec 1, 1988·Alcoholism, Clinical and Experimental Research·G E BjørneboeC A Drevon
Jan 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M A TalaminiA Hubbard
Oct 27, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H M KauffmannD Schrenk
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D RostD Keppler
Mar 20, 1999·Biochemical and Biophysical Research Communications·J NakamuraH Matsuda
Jan 3, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·E P Gallagher, K M Sheehy
Mar 7, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M SchmittD Häussinger
Mar 7, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G ZollnerM Trauner
Jun 11, 2003·Journal of Gastroenterology and Hepatology·Makoto HojoHajime Takikawa
Jun 19, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marieke G L ElferinkGeny M M Groothuis
Jun 19, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nathan J CherringtonCurtis D Klaassen
Mar 21, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A E M VickersR L Fisher
Jun 21, 2006·Free Radical Biology & Medicine·Shuichi SekineToshiharu Horie
Oct 24, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Daniel A J BowKim L R Brouwer
Nov 10, 2007·Cell Biology and Toxicology·Jun-ichi YamashiroToshiharu Horie
Jul 22, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Meng LiYurong Lai
Sep 13, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shuichi SekineToshiharu Horie
May 12, 2010·Biochemical Pharmacology·Kentaro YanoToshiharu Horie

❮ Previous
Next ❯

Citations

Aug 16, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mathieu VinkenVera Rogiers
May 4, 2011·Journal of Pharmaceutical Sciences·Tomoko SugiuraYukio Kato
Jun 12, 2013·Journal of Pharmaceutical Sciences·Kentaro YanoTakuo Ogihara
Oct 18, 2016·Journal of Pharmacological and Toxicological Methods·Attila Bátai-KonczosKatalin Jemnitz
Jun 4, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Akinori TakemuraKousei Ito
Sep 9, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·A L DzierlengaN J Cherrington
Jan 18, 2018·Journal of Biochemical and Molecular Toxicology·Anika L Dzierlenga, Nathan J Cherrington
Apr 6, 2018·Biological & Pharmaceutical Bulletin·Shun GuoYan Zhang
Dec 7, 2017·Current Medicinal Chemistry·Marcelo G RomaEnrique J Sánchez Pozzi
Jun 12, 2012·Journal of Pediatric Gastroenterology and Nutrition·Valerie ChampionDelphine Mitanchez
Nov 27, 2018·Case Reports in Nephrology·Rahaf NiaziOsama Safdar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.