Oxidative stress is involved in age-dependent spermatogenic damage of Immp2l mutant mice.

Free Radical Biology & Medicine
Sunil K GeorgeBaisong Lu

Abstract

Mitochondrial reactive oxygen species (ROS) have been implicated in spermatogenic damage, although direct in vivo evidence is lacking. We recently generated a mouse in which the inner mitochondrial membrane peptidase 2-like (Immp2l) gene is mutated. This Immp2l mutation impairs the processing of signal peptide sequences from mitochondrial cytochrome c₁ and glycerol phosphate dehydrogenase 2. The mitochondria from mutant mice generate elevated levels of superoxide ion, which causes age-dependent spermatogenic damage. Here we confirm age-dependent spermatogenic damage in a new cohort of mutants, which started at the age of 10.5 months. Compared with age-matched controls, protein carbonyl content was normal in testes of 2- to 5-month-old mutants, but significantly elevated in testes of 13-month-old mutants, indicating elevated oxidative stress in the testes at the time of impaired spermatogenesis. Testicular expression of superoxide dismutases was not different between control and mutant mice, whereas that of catalase was increased in young and old mutants. The expression of cytosolic glutathione peroxidase 4 (phospholipid hydroperoxidase) in testes was significantly reduced in 13-month-old mutants, concomitant with impaired sperm...Continue Reading

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Citations

Aug 29, 2012·Molecular and Cellular Biology·Yan JiaoBaisong Lu
Jun 19, 2013·Molecular and Cellular Endocrinology·João Ramalho-Santos, Sandra Amaral
Oct 18, 2016·Clinical Genetics·L A M DemainW G Newman
Apr 18, 2014·The Journal of Endocrinology·Changjie HanBaisong Lu
May 15, 2015·Nature Reviews. Molecular Cell Biology·Pedro M QuirósCarlos López-Otín
Jul 5, 2019·Environmental Science and Pollution Research International·Zeinab RafieeFereshteh Nejad-Dehbashi
Dec 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Chongjie YaoShimin Liu

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