Oxidative Stress Modulates the Expression Pattern of Peroxiredoxin-6 in Peripheral Blood Mononuclear Cells of Asthmatic Patients and Bronchial Epithelial Cells

Allergy, Asthma & Immunology Research
Hyun Jae ShimYou Sook Cho

Abstract

Reduction-oxidation reaction homeostasis is vital for regulating inflammatory conditions and its dysregulation may affect the pathogenesis of chronic airway inflammatory diseases such as asthma. Peroxiredoxin-6, an important intracellular anti-oxidant molecule, is reported to be highly expressed in the airways and lungs. The aim of this study was to analyze the expression pattern of peroxiredoxin-6 in the peripheral blood mononuclear cells (PBMCs) of asthmatic patients and in bronchial epithelial cells (BECs). The expression levels and modifications of peroxiredoxin-6 were evaluated in PBMCs from 22 asthmatic patients. Phosphorylated and acetylated peroxiredoxin-6 in hydrogen peroxide-treated human BECs was detected using immunoprecipitation analysis. The expression level of peroxiredoxin-6 was also investigated in BECs treated with hydrogen peroxide. Cycloheximide and proteasome inhibitors were used to determine whether peroxiredoxin-6 is degraded by proteasomes. Peroxiredoxin-6 expression was significantly reduced in the PBMCs of asthmatic patients compared to control subjects. Distinct modification patterns for peroxiredoxin-6 were observed in the PBMCs of asthmatic patients using 2-dimensional-electrophoresis. The levels of...Continue Reading

References

Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K TakeyamaJ A Nadel
May 12, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yan WangAron B Fisher
Feb 28, 2006·European Journal of Pharmacology·Irfan RahmanAruna Kode
May 9, 2006·Antioxidants & Redox Signaling·James R Stone, Suping Yang
Apr 17, 2007·Molecular Cell·Elizabeth A VealBrian A Morgan
May 1, 2008·The Journal of Biological Chemistry·Paul A GrimsrudDavid A Bernlohr
May 20, 2008·The Journal of Allergy and Clinical Immunology·Cansin SackesenOmer Kalayci
Jun 5, 2008·Antioxidants & Redox Signaling·Dunyaporn TrachoothamPeng Huang
Sep 23, 2008·The International Journal of Biochemistry & Cell Biology·Stephanie SpangeOliver H Krämer
Nov 27, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Thorsten EismannAlex B Lentsch
Jan 10, 2009·The Journal of Allergy and Clinical Immunology·Anne M FitzpatrickUNKNOWN Severe Asthma Research Program
Jul 25, 2009·Free Radical Biology & Medicine·David I Brown, Kathy K Griendling
Jul 29, 2009·Antioxidants & Redox Signaling·Suzy A A Comhair, Serpil C Erzurum
May 27, 2010·Parasite Immunology·M W RobinsonS Donnelly
Jul 2, 2010·Allergy, Asthma & Immunology Research·You Sook Cho, Hee-Bom Moon
Aug 19, 2010·Annals of the New York Academy of Sciences·Allen J Dozor
Dec 8, 2011·The Journal of Biological Chemistry·Sue Goo RheeSoo Han Bae
Nov 29, 2012·Annals of Thoracic Medicine·Asrar AhmadQayyum Husain
Oct 1, 2011·The World Allergy Organization Journal·Umit M SahinerOmer Kalayci
May 15, 2013·Molecular Immunology·Li ZuoKatherine S Salisbury
Jul 11, 2014·Redox Biology·Sylvie RiquierJean-Claude Drapier
Sep 19, 2015·Redox Biology·Marta PajaresAntonio Cuadrado
Dec 6, 2017·International Journal of Molecular Sciences·Michela Bullone, Jean-Pierre Lavoie

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Methods Mentioned

BETA
electrophoresis
immunoprecipitation
acetylation
bronchoalveolar
lavage

Software Mentioned

Excel
Multi Gauge

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