Oxidative stress: potential of distinct peroxide determination systems

Clinical Chemistry and Laboratory Medicine : CCLM
Meinrad LindschingerWillibald Wonisch

Abstract

When reactive oxygen species attack biological structures, peroxides, which are short-lived oxidative intermediates, are generated. We evaluated the potential of two different, commercially available peroxide activity assays (Pox-Act and d-ROMS) to see whether the results were associated with the clinical condition of subjects who were participating in a routine health care program. Furthermore, we determined the total antioxidant status (TAS) and the titer of autoantibodies against oxidized low-density lipoprotein (oLAb) to verify the hydroperoxide measurements. Subjects with medical conditions (hereafter referred to as patients) had significantly increased serum peroxide levels compared to healthy subjects. The d-ROMS kit indicated that 86% of subjects had an increased level of total peroxides. Although the assays had a significant correlation (p<0.001), 34% of the subjects had an increased total peroxide concentration in the Pox-Act assay that was clearly associated with clinical symptoms. Furthermore, the sensitivity of the Pox-Act assay was 35 times higher than that of the d-ROMS kit. In subjects with medical conditions, there was a trend toward a decreased TAS and a slightly increased oLAb titer in comparison to healthy s...Continue Reading

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Citations

May 19, 2006·European Journal of Applied Physiology·Mehmet Ibrahim HarmaOzcan Erel
May 19, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Eugenio Luigi Iorio, Maria Luisa Balestrieri
Dec 27, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Meinrad Lindschinger, Willibald Wonisch
Feb 27, 2007·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Mehmet AslanSahbettin Selek
Jun 20, 2006·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Mehmet Ibrahim HarmaOzcan Erel
Oct 11, 2005·Clinical Biochemistry·Ozcan Erel
Apr 30, 2014·Free Radical Research·K KilkP Hõrak
Feb 28, 2014·The Tohoku Journal of Experimental Medicine·Olaf StangerWillibald Wonisch
Sep 15, 2016·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Nathan A LewisCharles R Pedlar
Apr 4, 2012·The Aging Male : the Official Journal of the International Society for the Study of the Aging Male·Willibald WonischMeinrad Lindschinger

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