Oxidative stress responses in rainbow trout (Oncorhynchus mykiss) hepatocytes exposed to pro-oxidants and a complex environmental sample

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
E FarmenK E Tollefsen

Abstract

A wide range of pollutants in the aquatic environment have the capacity to induce toxic effects expressed as cellular oxidative stress. In the current study, the potential of an in vitro toxicity testing system was therefore investigated using rainbow trout (Oncorhynchus mykiss) hepatocytes to assess different endpoints of oxidative stress. The pro-oxidants CuSO(4) and paraquat were used as models for comparison to a complex environmental sample. Results following 6, 24, 48 and 96h exposure to different concentrations of these substances show cellular effects on intracellular ROS formation, glutathione levels and redox status, expression of the antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, gamma-glutamyl-cysteine synthetase (GCS) and thioredoxin, as well as cytotoxicity parameters. The most consistent effects (maximum values within brackets), observed in dose and time parameters for both model compounds and environmental sample, were the depletion of total glutathione (9.4% of control), induced levels of oxidized glutathione (695% of control), and gene expression regulation depicted relative to the control gene beta-actin of GCS mRNA (239% of control) and catalase (29% of con...Continue Reading

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Citations

May 16, 2012·Journal of Environmental Radioactivity·Lene Sørlie HeierBrit Salbu
Dec 14, 2011·Ecotoxicology·Kátia Cristina de Andrade WaldemarinAna Lúcia Kalinin
Jan 12, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Seonock Woo, Seungshic Yum
Sep 1, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mazyar Yazdani
Nov 30, 2014·Bulletin of Environmental Contamination and Toxicology·Mazyar YazdaniKetil Hylland
Jun 28, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Halina I FalfushynskaYoon Kwon Nam
Aug 4, 2016·Journal of Toxicology and Environmental Health. Part a·Louise Kiel JensenKnut Erik Tollefsen

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