Oxidative stress under low oxygen conditions triggers hyperflagellation and motility in the Antarctic bacterium Pseudomonas extremaustralis

Extremophiles : Life Under Extreme Conditions
Esmeralda C Solar VeneroNancy I López

Abstract

Reactive oxygen species and nitrogen species (ROS and RNS), produced in a wide range of physiological process even under low oxygen availability, are among the main stressors found in the environment. Strategies developed to combat them constitute key features in bacterial adaptability and survival. Pseudomonas extremaustralis is a metabolic versatile and stress resistant Antarctic bacterium, able to grow under different oxygen conditions. The present work explores the effect of oxidative stress under low oxygen conditions in P. extremaustralis, by combining RNA deep sequencing analysis and physiological studies. Cells grown under microaerobiosis exhibited more oxidative damage in macromolecules and lower survival rates than under aerobiosis. RNA-seq analysis showed an up-regulation of genes related with oxidative stress response, flagella, chemotaxis and biofilm formation while chaperones and cytochromes were down-regulated. Microaerobic cultures exposed to H2O2 also displayed a hyper-flagellated phenotype coupled with a high motility behavior. Moreover, cells that were subjected to oxidative stress presented increased biofilm formation. Altogether, our results suggest that a higher motile behavior and augmented capacity to fo...Continue Reading

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Citations

Sep 23, 2020·Environmental Science & Technology·Qiurong ZhangChengdong Zhang

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Datasets Mentioned

BETA
AHIP00000000
E-MTAB-7712

Methods Mentioned

BETA
PCR
transmission electron microscopy
RNA-seq

Software Mentioned

GraphPad Prism
KEGG
PRODORIC
Metacyc
ImageJ
Blast2GO
Biocyc
Rockhopper

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