Oxidative tyrosylation of HDL enhances the depletion of cellular cholesteryl esters by a mechanism independent of passive sterol desorption

Biochemistry
G A FrancisE L Bierman

Abstract

It is believed that HDL protects against atherosclerosis by removing excess cholesteryl esters from cells of the artery wall. Previous studies have suggested that HDL depletes cells of cholesteryl esters both by stimulating cholesterol efflux from the plasma membrane and by activating transport processes that divert cholesterol from the cholesteryl ester cycle, but it is unknown if these are independent processes. We previously found that HDL oxidized by tyrosyl radical has a markedly enhanced ability to promote the removal of cholesterol from cultured cells [Francis, G. A., et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 6631-6635]. Here we show that incubation of cholesterol-loaded human fibroblasts with low concentrations of tyrosylated HDL depleted cells of cholesteryl esters and increased cellular free cholesterol without increasing efflux of cholesterol into the medium as compared to incubation with untreated HDL. Cells preincubated with tyrosylated HDL and then exposed to a variety of cholesterol acceptors exhibited significantly higher rates of free cholesterol efflux than did cells preincubated with HDL. This effect was observed in the presence or absence of an inhibitor of acyl CoA:cholesterol acyltransferase (ACAT) a...Continue Reading

References

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Citations

May 21, 2005·Methods : a Companion to Methods in Enzymology·Emma I WaddingtonGordon A Francis
Jun 24, 1998·Biochimica Et Biophysica Acta·S Yokoyama
Nov 27, 2002·Vascular Pharmacology·Wendy JessupLen Kritharides
Jun 30, 2007·Antioxidants & Redox Signaling·Gerd Schmitz, Margot Grandl
May 14, 2005·Current Opinion in Lipidology·Shinji Yokoyama
Jan 5, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·D Bonnefont-RousselotJ Delattre
Apr 4, 2008·Journal of Atherosclerosis and Thrombosis·Dmitri SviridovPaul Nestel
Oct 8, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·G A Francis, R J Perry
Dec 7, 2010·Trends in Cardiovascular Medicine·Sima Allahverdian, Gordon A Francis
Sep 16, 2009·Free Radical Biology & Medicine·Jane A Leopold, Joseph Loscalzo
Dec 19, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Masashi UedaShinichi Mashiba
Jul 11, 2006·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Giuseppe Danilo NorataAlberico Luigi Catapano
Sep 15, 2005·Atherosclerosis·Gianna FerrettiGiovanna Curatola
Jul 1, 2008·Expert Opinion on Medical Diagnostics·Alan T Remaley, G Russell Warnick
Mar 1, 2006·Journal of Atherosclerosis and Thrombosis·Shinji Yokoyama
Mar 31, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Kristin L BowdenGordon A Francis
Nov 15, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Shinji Yokoyama
Jul 12, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Dawn L MacdonaldGordon A Francis
Sep 16, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Constanze BergtJay W Heinecke
Jun 19, 2003·The Journal of Biological Chemistry·Hong Y ChoiGordon A Francis
Oct 2, 2003·The Journal of Nutrition·Robert J HillstromSean M Lynch

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