Oxidised-HDL3 induces the expression of PAI-1 in human endothelial cells. Role of p38MAPK activation and mRNA stabilization

British Journal of Haematology
G D NorataPer Eriksson

Abstract

Modified lipoproteins have been suggested to modulate endothelial expression of plasminogen activator inhibitor-1 (PAI-1). As oxidized high-density lipoprotein (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the role of Ox-HDL3 on the expression of PAI-1. Ox-HDL3 but not native HDL3, increased PAI-1 mRNA expression in endothelial cells. Furthermore, PAI-1 antigen expression and activity increased in the supernatant of cells incubated with Ox-HDL3. The intracellular pathways involved in this effect were investigated. Ox-HDL3 activated both extracellular signal-regulated kinases (ERK) 1/2 and p38 mitogen-activated protein kinase (MAPK). Moreover, incubation with specific inhibitors of these kinases showed that p38MAPK was mainly involved in the Ox-HDL3-dependent PAI-1 induction. Transient transfection experiments suggested that none of the response elements in the proximal promoter (-804 to 17) were involved in Ox-HDL3-mediated PAI-1 expression. mRNA stability experiments showed that Ox-HDL3 increased the PAI-1 mRNA half-life. In summary, Ox-HDL3 induced PAI-1 mRNA expression and antigen release through a molecular mechanism involving MAPK activation...Continue Reading

References

Jan 1, 1992·The Journal of Clinical Investigation·J H ChinB B Hoffman
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y NaganoT Kita
Jan 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·L SironiE Tremoli
Jun 15, 2000·The New England Journal of Medicine·H P Kohler, P J Grant
Dec 16, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·Y ChenD J Schneider
Mar 7, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·A PirilloA L Catapano
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G MarscheE Malle
Nov 24, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·M TsumuraT Komoda
Mar 8, 2002·Atherosclerosis·Jerzy-Roch NoferArnold von Eckardstein
Sep 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Hiroyasu InoueToshiyuki Sasaguri
Mar 21, 2003·Biochemical and Biophysical Research Communications·Toshiyuki MatsunagaTsugikazu Komoda
Sep 13, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Laura CalabresiGuido Franceschini
Mar 5, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Giuseppe D NorataPer Eriksson

❮ Previous
Next ❯

Citations

Feb 2, 2008·Nature Reviews. Drug Discovery·Tisha Joy, Robert A Hegele
Aug 24, 2005·Antioxidants & Redox Signaling·Agnes Görlach
Mar 15, 2006·Current Opinion in Lipidology·Iveta DobrevaChristian Widmann
Jul 11, 2007·Current Opinion in Lipidology·Benjamin J AnsellAlan M Fogelman
Dec 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Yasushi MukaiJames K Liao
Feb 24, 2007·Vascular Health and Risk Management·Giuseppe D Norata, Alberico L Catapano
Jun 4, 2014·Cardiovascular Research·Marco van der StoepMiranda Van Eck
Jul 11, 2006·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Giuseppe Danilo NorataAlberico Luigi Catapano
Jun 19, 2013·Progress in Lipid Research·Kathleen M Botham, Caroline P D Wheeler-Jones
Jun 10, 2006·Circulation Research·Chieko MineoPhilip W Shaul
Aug 29, 2009·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Jian LiuXiufa Sun
Oct 23, 2008·Expert Review of Cardiovascular Therapy·Alan T RemaleyDmitri Sviridov
Oct 23, 2010·Expert Review of Molecular Diagnostics·Karin MengeleManfred Schmitt
Oct 3, 2019·Current Opinion in Lipidology·Fabrizia BonacinaGiuseppe D Norata
Jun 13, 2009·Current Opinion in Cardiology·Tisha Joy, Robert A Hegele
Dec 1, 2005·The Journal of Clinical Endocrinology and Metabolism·Giuseppe Danilo NorataAlberico Luigi Catapano
Jun 13, 2014·International Journal of Molecular Medicine·Xiang GaoChanglin Mei
Jan 23, 2020·International Journal of Molecular Sciences·Jacek RyszBeata Franczyk
Nov 30, 2018·International Journal of Molecular Sciences·Anna Reustle, Michael Torzewski
Oct 10, 2020·Molecular Aspects of Medicine·F BonacinaG D Norata
Sep 12, 2006·Free Radical Biology & Medicine·Anne Negre-SalvayreRobert Salvayre

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.