Oxidized cholesterol in oxidized low density lipoprotein may be responsible for the inhibition of LPS-induced nitric oxide production in macrophages

Atherosclerosis
S X LiuJ Wan

Abstract

Previous work has shown that oxidized low density lipoprotein (Ox-LDL) inhibited lipopolysacchride (LPS)-induced nitric oxide (NO) production in macrophages. In this paper, the role of different components of Ox-LDL in the inhibitory effect was studied by measuring nitrite in media. Ox-LDL inhibited LPS-induced NO production in macrophage cell line J774.A1. When compared with Ox-LDL, native and acetylated LDL had a lesser effect on NO production. Pre-clearance of lipid hydroperoxides (LOOH) in Ox-LDL and removal of soluble thiobarbituric acid reactive substances (TBARS) in Ox-LDL solution by dialysis had no influence on the inhibitory effect of Ox-LDL. The protein moiety of Ox-LDL had no effect on NO production, but the lipid moiety inhibited NO production to about the same extent as intact Ox-LDL. Linoleic acid and phosphatidylcholine, the main components of LDL lipid, whether oxidized separately or together, had no effect on NO production. However, if linoleic acid and cholesterol oxidized together, there was very strong inhibition of NO production. Cholesterol oxidized alone also had some inhibitory effect. These results suggest that oxidized cholesterol in Ox-LDL might be responsible for the inhibition of NO production in m...Continue Reading

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J SchuhR H Haschemeyer
Jan 1, 1992·The Journal of Clinical Investigation·J H ChinB B Hoffman
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Oct 1, 1992·Free Radical Biology & Medicine·H EsterbauerG Jürgens
Sep 1, 1992·The Journal of Clinical Investigation·J P CookeM E Billingham
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Mar 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·P M BathJ F Martin
May 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·I JialalS M Grundy
Jan 1, 1989·Free Radical Biology & Medicine·L L Smith, B H Johnson
Nov 30, 1988·Biochemical and Biophysical Research Communications·J B HibbsE M Rachlin
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jan 6, 1995·The Journal of Biological Chemistry·J K LiaoS L Clark
Jul 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H HughesJ R Guyton
Nov 1, 1995·Redox Report : Communications in Free Radical Research·S X LiuM J Sun

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Citations

Jan 27, 1999·Atherosclerosis·A J Brown, W Jessup
Jan 24, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Karin PerssonXi-Ming Yuan
Aug 23, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A VejuxG Lizard
Mar 11, 2004·American Journal of Physiology. Renal Physiology·I V SmirnovaM S Goligorsky
Jan 14, 2003·Nature Medicine·Sean B JosephPeter Tontonoz
Sep 4, 2007·The Journal of Biological Chemistry·Mark A BirrellMaria G Belvisi
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark A BirrellMaria G Belvisi
Jan 5, 2000·Physiological Reviews·G J Schroepfer

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