Abstract
The biological effects of oxidized LDL (oxLDL) may contribute to initiation and progression of the atherosclerotic process, and the association between cardiovascular disease and oxidation of LDL has been largely demonstrated. The objectives of this study were to establish the reference values of oxidative stress biomarkers in a young healthy Spanish population to determine the concentration of oxLDL and its relationship with lipid profile and with these biomarkers. oxLDL, F(2)-isoprostanes, protein carbonyls (PC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were determinate by ELISA in 72 healthy subjects. Antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) were carried out on a Hitachi 912 analyzer; lipid profile were assayed using automated systems (Cobas 711, Roche Diagnostics). All statistics were analyzed by using SPSS for Windows 15.0. SPSS Inc, Chicago, IL, USA. (Normal mean reference values): oxLDL (63.23 +/- 16.23 U/L), (Male/Female 68.06 +/- 17.69/58.39 +/- 13.6 U/L), F(2)-isoprostanes (2.26 +/- 0.9 microg/g creatinine), PC (0.34 +/- 0.15 nmol/mg), 8-OHdG (23.27 +/- 10.58 ng/ml), SOD (931.97 +/- 271.09 U/g Hb), GR (46.56 +/- 11.68 U/L), GPx (27.58 +/- 6.89 U/gHb ...Continue Reading
References
May 22, 1990·Biochimica Et Biophysica Acta·S ParthasarathyL G Fong
Dec 17, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S Orrenius
Jan 1, 1985·Advances in Enzyme Regulation·E Cadenas, H Sies
Feb 1, 1995·The Journal of Clinical Investigation·A D WatsonJ A Berliner
Dec 1, 1993·Atherosclerosis·M I MacknessP N Durrington
Sep 15, 1993·The Biochemical Journal·M I MacknessP N Durrington
Aug 7, 1998·Atherosclerosis·P Holvoet, D Collen
Oct 14, 1998·Circulation·P HolvoetD Collen
Nov 13, 1998·Clinical Biochemistry·J M HuangW Zhu
Aug 17, 2000·Current Opinion in Lipidology·M I MacknessB Mackness
Oct 14, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·S ToshimaR Nagai
Mar 10, 2001·Cell·C K Glass, J L Witztum
Apr 18, 2001·Circulation·S EharaA E Becker
May 23, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·P HolvoetF Van de Werf
Dec 25, 2002·Clinical Biochemistry·Metin OzataI Caglayan Ozdemir
Sep 6, 2003·Free Radical Biology & Medicine·Laura D KauffmanJill M Norris
Mar 12, 2004·Metabolism: Clinical and Experimental·Sachie TsuzuraKozo Hashimoto
Apr 3, 2004·Journal of Lipid Research·Mohamad NavabAlan M Fogelman
Apr 7, 2004·Journal of Biomedical Science·S MetsoTerho Lehtimäki
Jul 14, 2004·Diabetes/metabolism Research and Reviews·P G SchefferT Teerlink
Aug 31, 2004·Metabolism: Clinical and Experimental·Lilliam FloresE Esmatjes
Jul 12, 2005·Atherosclerosis·L J H van TitsA F H Stalenhoef
Jul 27, 2005·Circulation·Christa MeisingerWolfgang Koenig
Oct 12, 2005·Archives of Internal Medicine·Matteo CesariMarco Pahor
Nov 10, 2005·European Journal of Clinical Nutrition·B CerdáJ C Espín
Nov 18, 2005·Atherosclerosis·Jan GalleStefan Seibold
Feb 20, 2007·Current Medicinal Chemistry·Hirokazu Tsukahara
Jul 10, 2008·Scandinavian Journal of Clinical and Laboratory Investigation·V SigurdardottirJ Hulthe
Sep 20, 2008·Journal of Lipid Research·Leonard P van der ZwanPeter G Scheffer
Dec 3, 2008·Journal of Physiology and Biochemistry·M J Alcaraz-GarcíaS Parra
Citations
Apr 21, 2011·Lipids in Health and Disease·Kiriaque B F BarbosaJosefina Bressan
Dec 10, 2013·TheScientificWorldJournal·Filipa Mascarenhas-MeloFlávio Reis
Jan 9, 2013·Life Sciences·Krystyna PawlakDariusz Pawlak
Jun 1, 2012·Molecular Nutrition & Food Research·Joao Tomé-CarneiroJuan Carlos Espín
May 31, 2014·Oxidative Medicine and Cellular Longevity·Laura Di RenzoAntonino De Lorenzo
Jun 24, 2015·Mediators of Inflammation·Laura Di RenzoAntonino De Lorenzo
Mar 20, 2012·The Science of the Total Environment·Christian G Daughton
Apr 29, 2015·PloS One·Hongbin ShiKaijun Niu
Dec 21, 2012·European Journal of Preventive Cardiology·Helen Hermana M HermsdorffJ Alfredo Martínez
May 4, 2017·Environmental Research·N ArandaM Giralt
Apr 18, 2019·Nutrients·Biljana PokimicaMaria Glibetić
May 1, 2021·Nutrients·Desirée Victoria-MontesinosFrancisco Javier López-Román