PMID: 9657522Jul 10, 1998Paper

Oxidized LDL promotes vascular endothelial cell pinocytosis via a prooxidation mechanism

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
S E ChowJ K Chen

Abstract

Human low density lipoprotein (LDL) is prepared in the presence of antioxidants and is oxidized to different levels (measured by thiobarbituric acid reactive substance) with copper ion. The effects of unoxidized LDL and oxidized LDL (ox-LDL) on stress fiber formation, cell membrane ruffling, and pinocytosis (measured by [14C]sucrose uptake) in cultured human umbilical cord vein endothelial cells (EC) are compared. We show that at a concentration range of 100 to 200 microg cholesterol/ml, both unoxidized LDL and ox-LDL promote EC elongation and stress fiber formation, but the effect by the latter is more prominent when compared at the same dose range. In addition, ox-LDL also induces EC membrane ruffling and promotes pinocytosis. These effects are positively correlated with the extent of LDL oxidation and depend on the dose of ox-LDL. Ox-LDL-promoted membrane ruffling and pinocytosis are effectively blocked by brief preexposure of the cells to antioxidants. In contrast, stress fiber formation is not affected by antioxidant pretreatment. Although unoxidized LDL also promotes [14C]sucrose uptake, it is less potent than ox-LDL and significantly higher concentrations are required to produce a detectable effect. Unlike ox-LDL, unoxid...Continue Reading

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Citations

Jun 8, 2002·Toxicologic Pathology·Lilian Calderón-GarcidueñasBarry Rewcastle
Jun 13, 2002·American Journal of Physiology. Renal Physiology·Mark S SegalMehmet Koç
Jan 7, 2003·In Vitro Cellular & Developmental Biology. Animal·Shu-Er ChowJan-Kan Chen
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Oct 4, 2007·In Vitro Cellular & Developmental Biology. Animal·Pei-Ching KuoJan-Kan Chen
Jan 21, 2016·BMC Veterinary Research·Mariëlle van ZelstGeert P J Janssens
Feb 26, 2005·New Zealand Veterinary Journal·S E SimcockW H Hendriks
Aug 30, 2019·CPT: Pharmacometrics & Systems Pharmacology·Hannah M JonesRobert Webster
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C DesrumauxL Lagrost

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