Oxidized low density lipoprotein impairs endothelial progenitor cells by regulation of endothelial nitric oxide synthase.

Journal of Lipid Research
Feng Xia MaZhong Chao Han

Abstract

Oxidized low density lipoprotein (OxLDL) is one of the most important risk factors of cardiovascular disease. Here, we study the impact of OxLDL on endothelial progenitor cells (EPCs) and determine whether OxLDL affects EPCs by an inhibitory effect on endothelial nitric oxide synthase (eNOS). It was found that OxLDL decreased EPC survival and impaired its adhesive, migratory, and tube-formation capacities in a dose-dependent manner. However, all of the detrimental effects of OxLDL were attenuated by pretreatment of EPCs with lectin-like oxidized low density lipoprotein receptor (LOX-1) monoclonal antibody or l-arginine. Western blot analysis revealed that OxLDL dose-dependently decreased Akt phosphorylation and eNOS protein expression and increased LOX-1 protein expression. Furthermore, OxLDL caused a decrease in eNOS mRNA expression and an increase in LOX-1 mRNA expression. These data indicate that OxLDL inhibits EPC survival and impairs its function, and this action is attributable to an inhibitory effect on eNOS.

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Citations

Mar 1, 2012·Archives of Pharmacal Research·Kyeong-A KimOk-Nam Bae
May 7, 2009·Acta Pharmacologica Sinica·Feng-xia MaZhong-chao Han
Aug 18, 2010·Antioxidants & Redox Signaling·Felix Fleissner, Thomas Thum
Mar 11, 2010·Journal of Hypertension·Atushi NakanoTatsuya Sawamura
Jan 6, 2011·Exercise and Sport Sciences Reviews·Sarah WitkowskiJames M Hagberg
Jul 6, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Damir HamamdzicRobert L Wilensky
Sep 1, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Nathan T JenkinsJames M Hagberg
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