Oxidized phosphatidylcholines that modify proteins. Analysis by monoclonal antibody against oxidized low density lipoprotein.

The Journal of Biological Chemistry
H ItabeT Takano

Abstract

Oxidatively modified low density lipoprotein (OxLDL) is known to be involved in atherogenesis. We have previously developed a murine monoclonal antibody, FOH1a/DLH3, which recognized oxidatively modified lipoproteins as well as foam cells in human atherosclerotic lesions (Itabe, H., Takeshima, E., Iwasaki, H., Kimura, J., Yoshida, Y., Imanaka, T., and Takano, T. (1994) J. Biol. Chem. 269, 15274-15279). The antigen of this monoclonal antibody was formed by peroxidation of phosphatidylcholine (PC), and the antigenic oxidized PC (OxPC) derivatives are thought to form complexes with polypeptides including apolipoproteins. OxLDL was measured by a sensitive sandwich enzyme-linked immunosorbent assay using the monoclonal antibody and anti-human apolipoprotein B antibody, in which antigenic OxPC competed with OxLDL. When antigenic activities of PC analogs were tested by the competition assay, 1-palmitoyl-2-(9-oxononanoyl) PC (9-CHO PC) and the hydroperoxide of egg PC potently inhibited the detection of OxLDL. 1-Palmitoyl-2-linoleoyl PC was oxidized with ferrous ion and ascorbic acid, and the antigenic products were purified from the OxPC extracts on high pressure liquid chromatography columns and subsequently analyzed by laser desorpti...Continue Reading

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Citations

Oct 24, 2006·Amino Acids·A Trostchansky, H Rubbo
Nov 7, 2008·Clinical Reviews in Allergy & Immunology·Hiroyuki Itabe
Mar 4, 2009·Molecular Biotechnology·Arnis KuksisHeikki Kallio
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Sep 25, 2002·Clinical Biochemistry·Toru SuzukiUNKNOWN Clinical Trial of Oxidized LDL (CTOL) investigators
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Jan 10, 2003·Biological & Pharmaceutical Bulletin·Hiroyuki Itabe
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