Oxidizing reagent copper-o-phenanthroline is an open channel blocker of the vanilloid receptor TRPV1

Neuropharmacology
K TousovaV Vlachova

Abstract

The TRPV1 channel plays an important role in generating nociceptive signals in mammalian primary sensory neurons. It consists of 838 amino acids with six transmembrane segments (TM1-TM6), a pore-forming loop between TM5 and TM6 and N- and C- terminals located intracellularly. It is a homotetramer and forms a nonselective cationic channel that can be opened by capsaicin, weak acids and noxious heat. There are 18 cysteines (Cys), three of which are located on the extracellular side of the receptor in and around the region of the pore-forming loop. We report that the TRPV1 channel in transfected HEK293T cells and in cultured rat DRG neurons is blocked in the open state by an oxidizing agent Cu-o-phenanthroline complex (Cu:Phe). The effects of Cu:Phe are concentration dependent ( IC50 = 5.2 : 20.8 microm ) and fully reversible. Cu:Phe applied immediately before exposure to an acidic solution, capsaicin or noxious heat is without effect. Substitutions of the extracellular Cys residues (616, 621, 634) by glycine individually or together do not alter the blocking effects of Cu:Phe suggesting that disulfide cross-linking does not represent the underlying mechanism. It is suggested that the complex Cu:Phe, a bulky, positively charged mo...Continue Reading

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Citations

Oct 6, 2006·Pflügers Archiv : European journal of physiology·O P Hamill
Jan 11, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Elisa GhelfiBeatriz Gonzalez-Flecha
Dec 7, 2005·Circulation·Roberto Bolli, Ahmed Abdel-Latif
Oct 29, 2013·Antioxidants & Redox Signaling·Daisuke KozaiYasuo Mori
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Jan 25, 2006·British Journal of Pharmacology·Mariana Oana Popa, Holger Lerche
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Dec 3, 2014·Neuroscience Letters·Arafat NasserLisbeth B Møller
Jan 10, 2009·Der Anaesthesist·M Schmelz
Apr 15, 2006·Molecular Pharmacology·Klara SusankovaViktorie Vlachova

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