PMID: 7397145Jun 20, 1980

Oxonol-V as a probe of chromaffin granule membrane potentials

Biochimica Et Biophysica Acta
D Scherman, J P Henry

Abstract

The dye, oxonol-V (bis(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol), can be used to estimate the transmembrane potential of chromaffin granules. The potentials result either from a resting-state Donnan equilibrium (inside negative at pH 6.6) or from an ATP-driven proton pump. The fluorescence and absorption changes generated by ATP addition depended on the pH of the medium and the dye-to-vesicle ratio. Energization resulted in an increase in the number of oxonol-V binding sites, the new binding sites having the same dissociation constant. The rate of dye association was higher with resting than with energized chromaffin granules. The absorption change was associated with a red shift whereas the fluorescence change involved a quenching due to the increase in dye concentration on the membrane. The absorption and fluorescence changes varied linearly with the transmembrane potential difference when the interior potential was positive relative to the medium.

References

Jun 30, 1976·The Journal of Membrane Biology·A S Waggoner
Aug 4, 1970·Biochimica Et Biophysica Acta·E A Liberman, V P Skulachev
Jan 12, 1971·Biochimica Et Biophysica Acta·T FlatmarkK B Helle
Jan 1, 1974·The Journal of Membrane Biology·L B CohenC H Wang

Citations

Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D SchermanJ P Henry
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E Blumwald, R J Poole
Mar 1, 1987·Plant Physiology·K H Kaestner, H Sze
Oct 2, 1980·Biochimica Et Biophysica Acta·D Scherman, J P Henry
Mar 1, 1981·Bioscience Reports·C L Bashford
Oct 16, 1987·Biochimica Et Biophysica Acta·H J Apell, B Bersch
May 3, 2006·Lab on a Chip·Jan C T Eijkel, Albert van den Berg
Jan 1, 1987·Annals of the New York Academy of Sciences·J P HenryD Scherman
Jun 1, 1981·European Journal of Biochemistry·D Scherman, J P Henry
Jun 22, 1999·The Journal of Biological Chemistry·Y ChenS M Simon

Related Concepts

Oxonol V
Process of Absorption
Pentenes
Bos indicus
Chromaffin Granule
Chromaffin System
Fluorochromes
Isoxazoles
Resting Potentials
Norepinephrine, (+, -)-Isomer

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