Oxygen affinities of the hydrogenosome-containing protozoa Tritrichomonas foetus and Dasytricha ruminantium, and two aerobic protozoa, determined by bacterial bioluminescence

Journal of General Microbiology
D LloydA G Williams

Abstract

Oxygen-dependent bioluminescence of Photobacterium (Vibrio) fischeri was used to measure oxygen affinities of four protozoa. The aerobic organisms Acanthamoeba castellanii and Tetrahymena pyriformis showed apparent Km values for O2 of 0.42 and 2.43 microM respectively. The aerotolerant anaerobe Tritrichomonas foetus, and the more strictly anaerobic rumen ciliate Dasytricha ruminantium, both of which have hydrogenosomes, respired with apparent Km values of 1.08 and 1.70 microM-O2. We conclude that mitochondrial respiration is not the only process conferring on organisms a high affinity for O2.

Citations

May 1, 1986·Molecular and Biochemical Parasitology·N YarlettD Lloyd
Jan 15, 1987·Molecular and Biochemical Parasitology·T A PagetD Lloyd
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N YarlettA G Williams
Mar 21, 1998·International Journal for Parasitology·D M BrownP Upcroft
Oct 1, 2008·Biological Reviews of the Cambridge Philosophical Society·Tom Fenchel, B J Finlay
Feb 8, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jotaro IgarashiAriki Matsuoka
Mar 1, 1985·The British Journal of Nutrition·F J Lozeman, L P Milligan
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May 1, 1993·The Journal of Eukaryotic Microbiology·T A PagetE L Jarroll

Related Concepts

Amoeba genus
Phosphorescent Measurements
Oxygen Consumption
Photobacterium
Tetrahymena pyriformis
Tritrichomonas
Ciliata

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