May 10, 1976

Oxygen and carbon monoxide kinetics of Glycera dibranchiata monomeric hemoglobin

The Journal of Biological Chemistry
B SeamondsP D Smith

Abstract

Oxygen and carbon monoxide kinetics of Glycera dibranchiata monomeric hemoglobin have been studied using laser photolysis, air flash, and stopped flow techniques. The reactions of this hemoglobin with both ligands were found to be more rapid than the corresponding reactions involving myoglobin and were also biphasic in nature, the rate constants being approximately an order of magnitude different for the fast and slow phases in each case. No pH or hemoglobin concentration dependence of the pseudo-first order rate constants was apparent between pH 6 and 9 and in the concentration range of 1.25 to 40 muM heme. Both fast and slow pseudo-first order oxygen combination rate constants varied linearly with oxygen concentration between 16 and 1300 muM. A first order slow relaxation was also noted which was linearly dependent on heme concentration and inversely dependent on oxygen concentration. This reaction has been shown to be due to a replacement of oxygen by carbon monoxide. The presence of this reaction is a result of the high affinity of Glycera monomer for carbon monoxide as shown by the partition coefficient Mr = approximately 20,000 ana an equilibrium dissociation constant of the order L = 1.1 X 10(-9) M.

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Mentioned in this Paper

Dioxygen
Pseudo brand of pseudoephedrine
Plasma Protein Binding Capacity
Carbon Monoxide
Carboxyhemoglobin C
Hemoglobin Measurement
Eryhem
Glycera
Spectrophotometry
Myoglobin

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