Oxygen and mechanical ventilation impede the functional properties of resident lung mesenchymal stromal cells

PloS One
Alvaro G MoreiraShamimunisa B Mustafa

Abstract

Resident/endogenous mesenchymal stromal cells function to promote the normal development, growth, and repair of tissues. Following premature birth, the effects of routine neonatal care (e.g. oxygen support and mechanical ventilation) on the biological properties of lung endogenous mesenchymal stromal cells is (L-MSCs) is poorly understood. New Zealand white preterm rabbits were randomized into the following groups: (i) sacrificed at birth (Fetal), (ii) spontaneously breathing with 50% O2 for 4 hours (SB), or (iii) mechanical ventilation with 50% O2 for 4h (MV). At time of necropsy, L-MSCs were isolated, characterized, and compared. L-MSCs isolated from the MV group had decreased differentiation capacity, ability to form stem cell colonies, and expressed less vascular endothelial growth factor mRNA. Compared to Fetal L-MSCs, 98 and 458 genes were differentially expressed in the L-MSCs derived from the SB and MV groups, respectively. Gene ontology analysis revealed these genes were involved in key regulatory processes including cell cycle, cell division, and angiogenesis. Furthermore, the L-MSCs from the SB and MV groups had smaller mitochondria, nuclear changes, and distended endoplasmic reticula. Short-term hyperoxia/mechanical...Continue Reading

References

Oct 23, 1998·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·J PangH Yamamoto
Apr 2, 1999·Science·M F PittengerD R Marshak
Aug 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Aftab AhmadCarl W White
Jan 12, 2005·The Journal of Pathology·D G GrierT R Lappin
Feb 3, 2007·American Journal of Respiratory and Critical Care Medicine·Bernard Thébaud, Steven H Abman
Nov 9, 2007·The New England Journal of Medicine·Eugenio Baraldi, Marco Filippone
May 2, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·María José Martínez-LorenzoLuis Larrad
Aug 29, 2009·American Journal of Respiratory and Critical Care Medicine·Muhammad AslamStella Kourembanas
Aug 29, 2009·American Journal of Respiratory and Critical Care Medicine·Timothy van HaaftenBernard Thébaud
Mar 2, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Antonia P PopovaMarc B Hershenson
Dec 21, 2010·Current Opinion in Pediatrics·Alan H Jobe
Oct 15, 2011·Microscopy Research and Technique·Gabriella TetiMirella Falconi
Mar 16, 2013·American Journal of Respiratory and Critical Care Medicine·Ching Yong YickPeter J Sterk
Apr 23, 2013·Thorax·Hui-Leng Tan, Mark Rosenthal
Oct 16, 2013·Pediatric Research·Moses E Fung, Bernard Thébaud
Dec 12, 2013·BMC Research Notes·Tsung-Han LeeTao-Chen Lee
Dec 20, 2013·Antioxidants & Redox Signaling·Jennifer J P Collins, Bernard Thébaud
Jan 17, 2014·The Lancet. Respiratory Medicine·Janet StocksSamatha Sonnappa
Mar 13, 2014·Birth Defects Research. Part A, Clinical and Molecular Teratology·Jennifer J P Collins, Bernard Thébaud
Oct 19, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carl T D'Angio, Rita M Ryan
Nov 8, 2014·PloS One·Tao-Chen LeeMartin Hsiu-Chu Lin
Feb 15, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ana HouXindong Xue
Jul 17, 2015·Microscopy Research and Technique·Raluca ZaganescuCarmen Mihaela Mihu
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joanna KowalClotilde Théry
May 5, 2016·Molecular and Cellular Pediatrics·Marius A Möbius, Mario Rüdiger
Oct 23, 2016·Cell and Tissue Research·Megan O'Reilly, Bernard Thébaud
Feb 19, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ru-Jeng TengGirija G Konduri
Nov 11, 2017·The Cochrane Database of Systematic Reviews·Maria PierroRoger Soll
Dec 19, 2018·American Journal of Respiratory Cell and Molecular Biology·Marius A MöbiusBernard Thébaud

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Methods Mentioned

BETA
density gradient centrifugation
Feature Extraction
transmission electron microscopy

Software Mentioned

Agilent Feature Extraction
Ingenuity pathway
SPSS
GeneSpring
R
Applied
Ingenuity Pathway Analysis ( IPA
Ingenuity Pathway Analysis ( IPA )
GeneSpring GX
NIH Image J

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