Oxygen consumption and expression of the adenine nucleotide translocator in cells lacking mitochondrial DNA

Experimental Cell Research
Dominique LoiseauGeorges Stepien

Abstract

It has been shown previously that human rho degrees cells, deprived of mitochondrial DNA and consequently of functional oxidative phosphorylation, maintain a mitochondrial membrane potential, which is necessary for their growth. The goal of our study was to determine the precise origin of this membrane potential in three rho degrees cell lines originating from the human HepG2, 143B, and HeLa S3 cell lines. Residual cyanide-sensitive oxygen consumption suggests the persistence of residual mitochondrial respiratory chain activity, about 8% of that of the corresponding parental cells. The fluorescence emitted by the three rho degrees cell lines in the presence of a mitochondrial specific fluorochrome was partially reduced by a protonophore, suggesting the existence of a proton gradient. The mitochondrial membrane potential is maintained both by a residual proton gradient (up to 45 to 50% of the potential) and by other ion movements such as the glycolytic ATP(4-) to mitochondrial ADP(3-) exchange. The ANT2 gene, encoding isoform 2 of the adenine nucleotide translocator, is overexpressed in rho degrees HepG2 and 143B cells strongly dependent on glycolytic ATP synthesis, as compared to the corresponding parental cells, which present ...Continue Reading

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Citations

Oct 16, 2004·Molecular Carcinogenesis·Arnaud ChevrollierGeorges Stepien
Apr 26, 2008·Antioxidants & Redox Signaling·Giovanni Benard, Rodrigue Rossignol
Oct 21, 2016·PloS One·Mercedes Fernández-MorenoFrancisco J Blanco
Jan 24, 2007·Physiological Reviews·Jorgina SatrústeguiAraceli Del Arco
Jun 29, 2005·Current Genetics·Eugene G RikhvanovVictor K Voinikov
Dec 13, 2005·Journal of Bioenergetics and Biomembranes·Arnaud ChevrollierGeorges Stepien
Sep 16, 2003·Molecular Biology of the Cell·Carl D GajewskiGiovanni Manfredi
Feb 15, 2007·Journal of Cell Science·Giovanni BenardRodrigue Rossignol
Jun 5, 2004·Biochimica Et Biophysica Acta·Patries M HerstMichael V Berridge
Jan 18, 2006·Experimental Cell Research·Caroline JacquesPascal Reynier
Feb 3, 2007·Biochimica Et Biophysica Acta·Patries M Herst, Michael V Berridge
Mar 17, 2010·Biochimica Et Biophysica Acta·G BenardR Rossignol

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