Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XFe96 Extracellular Flux Analyzer.

Scientific Reports
G Du PreezV Wepener

Abstract

Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo effect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as a sublethal endpoint of toxicity. This protocol was tested by exposing 50 larval stage one C. elegans individuals for 48 h (at 20 °C) to different concentrations of two toxicants i.e. benzylcetyldimethylammonium chloride (BAC-C16) and cadmium (Cd). Following exposures, the oxygen consumption rate of the C. elegans individuals were measured using the high-throughput functionality of the Seahorse XFe96 Extracellular Flux Analyzer. Dose-response curves for BAC-C16 (R2 = 0.93; P = 0.001) and Cd (R2 = 0.98; P = 0.001) were created. Furthermore, a strong, positive correlation was evidenced between C. elegans oxygen consumption rate and a commonly used, ecologically relevant endpoint of toxicity (growth inhibition) for BAC-C16 (R2 = 0.93; P = 0.0001) and Cd (R2 = 0.91; P = 0.0001). The data presented in this study show that C. elegans oxygen consumption rate can be used as a promising functional measurement of toxicity.

References

Nov 24, 2001·Mechanisms of Ageing and Development·Bart P BraeckmanJacques R Vanfleteren
Nov 12, 2002·Mechanisms of Ageing and Development·Bart P BraeckmanJacques R Vanfleteren
Jul 29, 2003·Aging Cell·Bart P BraeckmanJacques R Vanfleteren
Apr 6, 2005·Biochemical and Biophysical Research Communications·Hitoshi SudaNaoaki Ishii
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Theresa Stiernagle
Nov 14, 2008·Environmental Toxicology and Chemistry·Katherine SchouestDmitri Papkovsky
May 5, 2009·Toxicology and Applied Pharmacology·Lars Järup, Agneta Akesson
Mar 9, 2010·Toxicology and Applied Pharmacology·Windy A BoydJonathan H Freedman
Oct 22, 2011·Environmental Science & Technology·S HössW Traunspurger
Nov 1, 2011·Environmental Pollution·Céline VellingerPhilippe Usseglio-Polatera
May 1, 2012·Environmental Toxicology and Chemistry·Sebastian HössHans-Jürgen Pluta
Apr 1, 2012·International Journal of Endocrinology and Metabolism·Asghar Ghasemi, Saleh Zahediasl
Dec 18, 2013·Advanced Drug Delivery Reviews·Linda P O'ReillyStephen C Pak
Jan 23, 2015·Environmental Science & Technology·Sang-Kyu JungWeiwei Zhong
Aug 4, 2015·Environmental Science and Pollution Research International·Annette BérardYvan Capowiez
Aug 21, 2015·Proceedings. Biological Sciences·Sravanti Uppaluri, Clifford P Brangwynne
Sep 17, 2015·Brain : a Journal of Neurology·Andreia Teixeira-CastroPatrícia Maciel
May 25, 2016·Toxicology·Des W ConnellVibha Verma
Jul 23, 2016·Journal of Applied Toxicology : JAT·Piper Reid Hunt
Sep 2, 2016·Nature Protocols·Mandy KoopmanRiekelt H Houtkooper
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Itamar LevOded Rechavi
Nov 7, 2019·The Science of the Total Environment·Adam Bownik

❮ Previous
Next ❯

Software Mentioned

Agilent Seahorse Wave
STAT
SAS
ToxRat Professional
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.