PMID: 9161846Mar 1, 1997Paper

Oxygen-dependent fragmentation of cellular DNA by nitric oxide

Free Radical Research
M YabukiKozo Utsumi

Abstract

Although active oxygen species and related metabolites, such as nitric oxide (NO), have been postulated to play important roles in the apoptosis of various cells, a precise mechanism leading to cell death remains to be elucidated. Recently we found that the lifetime of NO depends greatly on the concentration of environmental oxygen and that NO reversibly inhibits mitochondrial respiration and ATP synthesis; the inhibitory effect is stronger at physiologically low oxygen tension than under atmospheric conditions (Arch. Biochem. Biophys. 323, 27-32, 1995). The present work describes the effects of the NO-generating agent, 1-hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene (NOC 18) and oxygen tension on the respiration, ATP synthesis and apoptosis of HL-60 cells. When respiration was inhibited by NOC 18, cellular ATP levels decreased significantly and DNA fragmentation was elicited. Both events were enhanced by decreasing oxygen tension and suppressed by adding NO-trapping agents, such as 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO) and oxyhemoglobin. The fragmentation of cellular DNA was inhibited in a dose dependent manner by herbimycin A, a tyrosine kinase inhibitor. Fragmentation of the DNA of...Continue Reading

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Citations

Sep 6, 2002·Pharmacology, Biochemistry, and Behavior·Jun YangJames D Adams
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·M TsuchiyaM Inoue
Aug 26, 1999·Cell Structure and Function·S OkadaK Utsumi
Mar 4, 2000·Cell Structure and Function·R IshisakaK Utsumi
Oct 29, 2002·Bioscience, Biotechnology, and Biochemistry·Rumi IshisakaToshihiko Utsumi
Aug 14, 1998·Free Radical Research·H NakaharaK Utsumi
Sep 7, 2004·Free Radical Research·Shikibu MuranakaKozo Utsumi
Dec 23, 2006·Neurobiology of Aging·Kelley A FosterDennis A Turner
Nov 30, 2006·Redox Report : Communications in Free Radical Research·Tiffany A Reiter
Mar 8, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·T WeiW Xin
Jul 9, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·C Pascual, K Reinhart
Nov 4, 2000·Stroke; a Journal of Cerebral Circulation·K SampeiP D Hurn
May 7, 1999·Free Radical Research·Y TakeharaK Utsumi

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