PMID: 9546173Apr 18, 1998Paper

Oxygen-dependent regulation of the expression of the catalase gene katA of Lactobacillus sakei LTH677

Applied and Environmental Microbiology
Christian HertelW P Hammes

Abstract

The catalase gene katA of Lactobacillus sakei LTH677 was cloned and expressed in Escherichia coli UM2, Lactobacillus casei LK1, and Lactobacillus curvatus LTH1432. The last host is a catalase-deficient plasmid-cured derivative of a starter organism used in meat fermentation. The regulation of katA expression was found to be the same in L. sakei LTH677 and the recombinant strains. The addition of H2O2 to anaerobic cultures, as well as a switch to aerobic conditions, resulted in a strong increase in KatA activity. The expression was investigated in more detail with L. sakei LTH677 and L. curvatus LTH4002. The recombinant strain LTH4002 did not accumulate H2O2 under glucose-limited aerobic conditions and remained viable in the stationary phase. Under inductive conditions, the katA-specific mRNA and the apoenzyme were synthesized de novo. Deletion derivatives of the katA promoter were produced, and the regulatory response was investigated by fusion to the beta-glucuronidase reporter gene gusA and expression in L. sakei LTH677. The fact that gene expression was subject to induction was confirmed at the level of transcription and protein synthesis. A small putative regulatory sequence of at least 25 bp was identified located upstream...Continue Reading

References

Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Feb 1, 1991·International Journal of Food Microbiology·G WolfW P Hammes
Dec 1, 1987·Journal of Bacteriology·P C Loewen, J Switala
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
Sep 1, 1995·FEMS Microbiology Letters·H E Schellhorn
Jan 1, 1994·Antonie van Leeuwenhoek·S Spiro
Feb 11, 1993·Nucleic Acids Research·A S Irvine, J R Guest
Dec 1, 1995·Microbiology·M ObstW P Hammes
Nov 22, 1996·The Journal of Biological Chemistry·T IgarashiK Tanaka
Jun 15, 1997·FEMS Microbiology Letters·R P Ross, A Claiborne
Jan 1, 1994·Meat Science·W P Hammes, H J Knauf
May 1, 1996·Microbiology·Françoise BerthierFrançoise Morel-Deville

❮ Previous
Next ❯

Citations

Jul 16, 2003·Letters in Applied Microbiology·L AxelssonK Naterstad
Jul 19, 2020·Applied and Environmental Microbiology·Emilie VerplaetseMarie-Christine Champomier-Vergès
Jan 20, 2004·Proteomics·Maria De Angelis, Marco Gobbetti
Nov 8, 2005·Nature Biotechnology·Stéphane ChaillouMonique Zagorec
Apr 25, 2020·Bioscience of Microbiota, Food and Health·Shingo NarakiYasuko Sasaki
Mar 5, 2020·Applied Microbiology and Biotechnology·Ángela Peirotén, José M Landete
Sep 15, 2001·Journal of Applied Microbiology·C BarrièreR Talon
Jan 9, 2004·Applied and Environmental Microbiology·Hikmate AbriouelCharles M A P Franz
Jun 27, 2001·Applied and Environmental Microbiology·S ShimaR K Thauer
May 7, 2016·International Journal of Systematic and Evolutionary Microbiology·Jaewoo YoonHiroaki Kasai
Feb 29, 2016·Food Science and Biotechnology·Carla María Blanco-LizarazoAdriana Llorente-Bousquets
Mar 4, 2011·Molecular Genetics and Genomics : MGG·O Ludvig NyquistIngolf F Nes
Sep 8, 2017·Microorganisms·Monique Zagorec, Marie-Christine Champomier-Vergès
Apr 28, 2018·International Journal of Systematic and Evolutionary Microbiology·Jaewoo Yoon, Dong-Hee Kang
Jan 30, 1999·Applied and Environmental Microbiology·S FaddaF Toldrá
Jul 6, 2020·International Journal of Food Microbiology·Dorothee JanßenRudi F Vogel
Jul 17, 2020·Journal of Agricultural and Food Chemistry·Nuanyi LiangMichael G Gänzle
Jun 29, 1999·Current Opinion in Microbiology·W M de Vos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.