Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation

Journal of Vascular Research
Mads RiemannChristian Torp-Pedersen

Abstract

A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smooth muscle cell layers are necessary components in the signaling pathway. The study was performed in mouse cremaster muscle superfused with Krebs buffer. A section of the capillary bed was then superfused with human red blood cell suspension equilibrated with either 95% nitrogen or 95% oxygen, and 5% carbon dioxide. The superfusions caused a 12.9 ± 2.4% (p < 0.01) dilation and a 12.3 ± 2.7% (p < 0.01) constriction of the supplying non-exposed arteriole. Vasomotor responses could be detected 1 mm upstream of the stimulation site. The responses to hypoxia and hyperoxia were not affected by inhibition of nitric oxide (NO) synthases by L-NAME. Damage to the wall of an intervening segment of the arteriole abolished upstream changes. The method is capable of changing the oxygen tension locally in a membranous tissue and elicits NO-independent local and upstream vasomotor responses. Upstream responses were transmitted by a conducted ...Continue Reading

Citations

May 24, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Clare E ThornAngela C Shore
Dec 27, 2011·Critical Reviews in Biomedical Engineering·Donald G BuerkDov Jaron
Dec 27, 2011·Critical Reviews in Biomedical Engineering·Adam KapelaNikolaos M Tsoukias
Sep 9, 2015·Anaesthesia·A M E Spoelstra-de ManY M Smulders
Nov 30, 2014·Microvascular Research·Diego Orbegozo CortésDaniel De Backer
Jun 22, 2016·The Journal of Physiology·William F Jackson
Apr 15, 2017·Intensive Care Medicine Experimental·Ilias AttayeAngelique M E Spoelstra-de Man
Aug 5, 2018·Critical Care : the Official Journal of the Critical Care Forum·Bob SmitAngelique M E Spoelstra-de Man

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